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Scientific article
English

Characterization of an antibody-drug conjugate by hydrophilic interaction chromatography coupled to mass spectrometry

Published inJournal of Chromatography. B, vol. 1080, p. 37-41
Publication date2018
Abstract

Brentuximab vedotin (Adcetris) is a cysteine-linked antibody-drug conjugate (ADC) used in the treatment of Hodgkin lymphoma (HL) and systemic anaplastic large cell lymphoma (ALCL). In this study, the drug payload and glycan modifications of this ADC were simultaneously characterized using a unique LC-MS middle-up analysis, involving hydrophilic interaction chromatography (HILIC). This work demonstrates that HILIC is an effective and complementary analytical technique to reversed phase liquid chromatography (RPLC) for subunitlevel characterization of immuno-conjugates.

Keywords
  • Antibody-drug conjugate
  • Hydrophilic interaction chromatography
  • High-resolution mass spectrometry
  • Post-translational modifications
  • Glycans profiling
Citation (ISO format)
D’ATRI, Valentina et al. Characterization of an antibody-drug conjugate by hydrophilic interaction chromatography coupled to mass spectrometry. In: Journal of Chromatography. B, 2018, vol. 1080, p. 37–41. doi: 10.1016/j.jchromb.2018.02.026
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Article (Published version)
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Identifiers
ISSN of the journal1570-0232
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Technical informations

Creation2018/02/28 10:55:00
First validation2018/02/28 10:55:00
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