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Title

Characterization of an antibody-drug conjugate by hydrophilic interaction chromatography coupled to mass spectrometry

Authors
Stoll, Dwight
Lauber, Matthew
Beck, Alain
Published in Journal of Chromatography. B. 2018, vol. 1080, p. 37-41
Abstract Brentuximab vedotin (Adcetris) is a cysteine-linked antibody-drug conjugate (ADC) used in the treatment of Hodgkin lymphoma (HL) and systemic anaplastic large cell lymphoma (ALCL). In this study, the drug payload and glycan modifications of this ADC were simultaneously characterized using a unique LC-MS middle-up analysis, involving hydrophilic interaction chromatography (HILIC). This work demonstrates that HILIC is an effective and complementary analytical technique to reversed phase liquid chromatography (RPLC) for subunitlevel characterization of immuno-conjugates.
Keywords Antibody-drug conjugateHydrophilic interaction chromatographyHigh-resolution mass spectrometryPost-translational modificationsGlycans profiling
Identifiers
PMID: 29477065
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Research group Sciences analytiques
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D'ATRI, Valentina et al. Characterization of an antibody-drug conjugate by hydrophilic interaction chromatography coupled to mass spectrometry. In: Journal of Chromatography. B, 2018, vol. 1080, p. 37-41. https://archive-ouverte.unige.ch/unige:102599

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Deposited on : 2018-02-28

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