UNIGE document Scientific Article
previous document  unige:101953  next document
add to browser collection

Separation of Chlorins and Carotenoids in Capillary Electrophoresis

Published in Journal of Chromatographic Science. 2007, vol. 45, no. 9, p. 593-599
Abstract The present study reports the investigation of capillary electrophoresis (CE) for the separation of the photosynthetic pigments (chlorophyll derivatives as well as carotenoids) together. Various CE methods, such as micellar electrokinetic chromatography, capillary electrokinetic chromatography, and nonaqueous capillary electrophoresis (NACE) are tested, with coated and uncoated capillary columns to evaluate optimal separation conditions using diode array detection. The effect of different type and composition of organic solvents and surfactants on the separation is discussed. Detection limits are found in the range of 1.14–2.45 ppm. According to the system suitability results, the most effective separation is observed using NACE with Aliquat 336 as cationic surfactant in coated capillary and mixture of MeOH-ACN-THF (5:4:1, v/v/v) as solvent. Quantitative evolution is investigated, and recovery percentage values are found to be 96.7–102%.
Full text
Article (Published version) (297 Kb) - public document Free access
Research group Open Access - Licence nationale Oxford University Press
(ISO format)
BELIN-KAVRAN, Gamze, GULACAR, Fazil. Separation of Chlorins and Carotenoids in Capillary Electrophoresis. In: Journal of Chromatographic Science, 2007, vol. 45, n° 9, p. 593-599. https://archive-ouverte.unige.ch/unige:101953

95 hits



Deposited on : 2018-02-07

Export document
Format :
Citation style :