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Scientific article
English

T-type alpha 1H Ca2+ channels are involved in Ca2+ signaling during terminal differentiation (fusion) of human myoblasts

Publication date2000
Abstract

Mechanisms underlying Ca(2+) signaling during human myoblast terminal differentiation were studied using cell cultures. We found that T-type Ca(2+) channels (T-channels) are expressed in myoblasts just before fusion. Their inhibition by amiloride or Ni(2+) suppresses fusion and prevents an intracellular Ca(2+) concentration increase normally observed at the onset of fusion. The use of antisense oligonucleotides indicates that the functional T-channels are formed by alpha1H subunits. At hyperpolarized potentials, these channels allow a window current sufficient to increase [Ca(2+)](i). As hyperpolarization is a prerequisite to myoblast fusion, we conclude that the Ca(2+) signal required for fusion is produced when the resting potential enters the T-channel window. A similar mechanism could operate in other cell types of which differentiation implicates membrane hyperpolarization.

Keywords
  • Calcium/ metabolism
  • Calcium Channels, T-Type/ metabolism
  • Cell Differentiation
  • Cells, Cultured
  • Humans
  • Ion Transport
  • Muscle, Skeletal/cytology/ metabolism
  • Signal Transduction
Citation (ISO format)
BIJLENGA, P. et al. T-type alpha 1H Ca2+ channels are involved in Ca2+ signaling during terminal differentiation (fusion) of human myoblasts. In: Proceedings of the National Academy of Sciences of the United States of America, 2000, vol. 97, n° 13, p. 7627–7632. doi: 10.1073/pnas.97.13.7627
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ISSN of the journal0027-8424
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