

Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC16596/pdf/pq007627.pdf
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T-type alpha 1H Ca2+ channels are involved in Ca2+ signaling during terminal differentiation (fusion) of human myoblasts |
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Published in | Proceedings of the National Academy of Sciences. 2000, vol. 97, no. 13, p. 7627-7632 | |
Abstract | Mechanisms underlying Ca(2+) signaling during human myoblast terminal differentiation were studied using cell cultures. We found that T-type Ca(2+) channels (T-channels) are expressed in myoblasts just before fusion. Their inhibition by amiloride or Ni(2+) suppresses fusion and prevents an intracellular Ca(2+) concentration increase normally observed at the onset of fusion. The use of antisense oligonucleotides indicates that the functional T-channels are formed by alpha1H subunits. At hyperpolarized potentials, these channels allow a window current sufficient to increase [Ca(2+)](i). As hyperpolarization is a prerequisite to myoblast fusion, we conclude that the Ca(2+) signal required for fusion is produced when the resting potential enters the T-channel window. A similar mechanism could operate in other cell types of which differentiation implicates membrane hyperpolarization. | |
Keywords | Calcium/ metabolism — Calcium Channels, T-Type/ metabolism — Cell Differentiation — Cells, Cultured — Humans — Ion Transport — Muscle, Skeletal/cytology/ metabolism — Signal Transduction | |
Identifiers | PMID: 10861024 | |
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![]() ![]() Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC16596/pdf/pq007627.pdf |
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Citation (ISO format) | BIJLENGA, P. et al. T-type alpha 1H Ca2+ channels are involved in Ca2+ signaling during terminal differentiation (fusion) of human myoblasts. In: Proceedings of the National Academy of Sciences, 2000, vol. 97, n° 13, p. 7627-7632. https://archive-ouverte.unige.ch/unige:10191 |