T-type alpha 1H Ca2+ channels are involved in Ca2+ signaling during terminal differentiation (fusion) of human myoblasts

Bijlenga, P.; Liu, J. H.; Espinos, E.; Haenggeli, Charles-Antoine; Fischer-Lougheed, Jacqueline; Bader, Charles; Bernheim, Laurent

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Title		T-type alpha 1H Ca2+ channels are involved in Ca2+ signaling during terminal differentiation (fusion) of human myoblasts
Authors		Bijlenga, P. Liu, J. H. Espinos, E. Haenggeli, Charles-Antoine Fischer-Lougheed, Jacqueline Bader, Charles Bernheim, Laurent
Published in		Proceedings of the National Academy of Sciences. 2000, vol. 97, no. 13, p. 7627-7632
Abstract		Mechanisms underlying Ca(2+) signaling during human myoblast terminal differentiation were studied using cell cultures. We found that T-type Ca(2+) channels (T-channels) are expressed in myoblasts just before fusion. Their inhibition by amiloride or Ni(2+) suppresses fusion and prevents an intracellular Ca(2+) concentration increase normally observed at the onset of fusion. The use of antisense oligonucleotides indicates that the functional T-channels are formed by alpha1H subunits. At hyperpolarized potentials, these channels allow a window current sufficient to increase [Ca(2+)](i). As hyperpolarization is a prerequisite to myoblast fusion, we conclude that the Ca(2+) signal required for fusion is produced when the resting potential enters the T-channel window. A similar mechanism could operate in other cell types of which differentiation implicates membrane hyperpolarization.
Keywords		Calcium/ metabolism — Calcium Channels, T-Type/ metabolism — Cell Differentiation — Cells, Cultured — Humans — Ion Transport — Muscle, Skeletal/cytology/ metabolism — Signal Transduction
Identifiers		DOI: 10.1073/pnas.97.13.7627 PMID: 10861024
Full text		Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC16596/pdf/pq007627.pdf
Structures		Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales Faculté de médecine / Section de médecine clinique / Département de pédiatrie
Citation (ISO format)		BIJLENGA, P. et al. T-type alpha 1H Ca2+ channels are involved in Ca2+ signaling during terminal differentiation (fusion) of human myoblasts. In: Proceedings of the National Academy of Sciences, 2000, vol. 97, n° 13, p. 7627-7632. https://archive-ouverte.unige.ch/unige:10191