Scientific article

Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation

Published inScience, vol. 276, no. 5321, p. 2042-2045
Publication date1997

Long-term potentiation (LTP), a cellular model of learning and memory, requires calcium-dependent protein kinases. Induction of LTP increased the phosphorus-32 labeling of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPA-Rs), which mediate rapid excitatory synaptic transmission. This AMPA-R phosphorylation appeared to be catalyzed by Ca2+- and calmodulin-dependent protein kinase II (CaM-KII): (i) it correlated with the activation and autophosphorylation of CaM-KII, (ii) it was blocked by the CaM-KII inhibitor KN-62, and (iii) its phosphorus-32 peptide map was the same as that of GluR1 coexpressed with activated CaM-KII in HEK-293 cells. This covalent modulation of AMPA-Rs in LTP provides a postsynaptic molecular mechanism for synaptic plasticity.

  • Animals
  • Calcium/metabolism
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2
  • Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors/ metabolism
  • Cell Line
  • Enzyme Inhibitors/pharmacology
  • Excitatory Amino Acid Antagonists/pharmacology
  • Hippocampus/ metabolism
  • Humans
  • Long-Term Potentiation/drug effects
  • Male
  • Peptide Mapping
  • Phosphorylation
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, AMPA/ metabolism
  • Synaptic Transmission/drug effects
Citation (ISO format)
BARRIA, A. et al. Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation. In: Science, 1997, vol. 276, n° 5321, p. 2042–2045. doi: 10.1126/science.276.5321.2042
Updates (1)
ISSN of the journal0036-8075

Technical informations

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