UNIGE document Scientific Article
previous document  unige:101538  next document
add to browser collection

First insights into structure-function relationships of alkylglycerol monooxygenase

Watschinger, Katrin
Fuchs, Julian E.
Yarov-Yarovoy, Vladimir
Keller, Markus A.
Golderer, Georg
Hermetter, Albin
Werner-Felmayer, Gabriele
show hidden authors show all authors [1 - 9]
Published in Pteridines. 2013, vol. 24, no. 1, p. 99-103
Collection Open Access - Licence nationale de Gruyter
Abstract Alkylglycerol monooxygenase is a tetrahydrobiopterin-dependent enzyme that cleaves the O-alkyl-bond of alkylglycerols. It is an exceptionally unstable, hydrophobic membrane protein which has never been purified in active form. Recently, we were able to identify the sequence of alkylglycerol monooxygenase. TMEM195, the gene coding for alkylglycerol monooxygenase, belongs to the fatty acid hydroxylases, a family of integral membrane enzymes which have an 8-histidine motif crucial for catalysis. Mutation of each of these residues resulted in a complete loss of activity. We now extended the mutational analysis to another 25 residues and identified three further residues conserved throughout all members of the fatty acid hydroxylases which are essential for alkylglycerol monooxygenase activity. Furthermore, mutation of a specific glutamate resulted in an 18-fold decreased affinity of the protein to tetrahydrobiopterin, strongly indicating a potential important role in cofactor interaction. A glutamate residue in a comparable amino acid surrounding had already been shown to be responsible for tetrahydrobiopterin binding in the aromatic amino acid hydroxylases. Ab initio modelling of the enzyme yielded a structural model for the central part of alkylglycerol monooxygenase where all essential residues identified by mutational analysis are in close spatial vicinity, thereby defining the potential catalytic site of this enzyme.
Full text
(ISO format)
WATSCHINGER, Katrin et al. First insights into structure-function relationships of alkylglycerol monooxygenase. In: Pteridines, 2013, vol. 24, n° 1, p. 99-103. doi: 10.1515/pterid-2013-0007 https://archive-ouverte.unige.ch/unige:101538

342 hits



Deposited on : 2018-01-22

Export document
Format :
Citation style :