Electrophysiology and lucifer yellow injection of nucleus gigantocellularis neurones in an isolated and perfused guinea pig brain in vitro
|Published in||Neuroscience Letters. 1990, vol. 120, no. 1, p. 5-8|
|Abstract||Intracellular recordings from nucleus gigantocellularis (NGC) neurones were obtained in isolated and perfused whole brains of guinea pigs in vitro. A majority of cells (90%) were characterized by an action potential of short duration (0.3 ms) followed first by a fast and then by a slower afterhyperpolarization (AHP). Their firing pattern was mostly irregular. These cells were shown to have high threshold calcium spikes and plateau potentials. The other cell type represented only 10% of the recorded cells in the NGC. It was characterized by a wider (0.6 ms) action potential, a large single AHP, the presence of a transient rectification presumably due to an A-current and a rather regular resting discharge. Using Lucifer yellow injections in brainstem slices, both cell types were shown to correspond to gigantocellular neurones.|
|Keywords||Action Potentials/drug effects — Animals — Brain/cytology/ physiology — Electrophysiology/methods — Fluorescent Dyes — Guinea Pigs — Isoquinolines — Microelectrodes — Neurons/cytology/drug effects/ physiology — Tetrodotoxin/pharmacology|
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