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Electrophysiology and lucifer yellow injection of nucleus gigantocellularis neurones in an isolated and perfused guinea pig brain in vitro

Khateb, A.
Published in Neuroscience Letters. 1990, vol. 120, no. 1, p. 5-8
Abstract Intracellular recordings from nucleus gigantocellularis (NGC) neurones were obtained in isolated and perfused whole brains of guinea pigs in vitro. A majority of cells (90%) were characterized by an action potential of short duration (0.3 ms) followed first by a fast and then by a slower afterhyperpolarization (AHP). Their firing pattern was mostly irregular. These cells were shown to have high threshold calcium spikes and plateau potentials. The other cell type represented only 10% of the recorded cells in the NGC. It was characterized by a wider (0.6 ms) action potential, a large single AHP, the presence of a transient rectification presumably due to an A-current and a rather regular resting discharge. Using Lucifer yellow injections in brainstem slices, both cell types were shown to correspond to gigantocellular neurones.
Keywords Action Potentials/drug effectsAnimalsBrain/cytology/ physiologyElectrophysiology/methodsFluorescent DyesGuinea PigsIsoquinolinesMicroelectrodesNeurons/cytology/drug effects/ physiologyTetrodotoxin/pharmacology
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PMID: 2293091

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Deposited on : 2010-08-06

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