Scientific article

The isolated and perfused brain of the guinea-pig in vitro

Published inEuropean journal of neuroscience, vol. 5, no. 7, p. 915-926
Publication date1993

We describe here an isolated and perfused in vitro adult guinea-pig whole brain preparation which is an extension of the previously described in vitro brainstem-cerebellum preparation. Viability was tested by the analysis of trans-synaptic responses along the visual pathways following the electrical stimulation of the optic nerve or the optic radiations. The evoked field potentials were recorded in the dorsal lateral geniculate, the superior colliculus and the visual cortex. The distribution of extracellular currents was studied using current source density analysis, in order to determine the amplitude, time course and spatial organization of the synaptic activity at these sites. The study indicates that field potentials were very similar to those described in vivo. These data demonstrate the survival of a complex adult sensory system in vitro and suggest that this preparation can be used for the analysis of multisynaptic circuits in the mammalian brain.

  • Animals
  • Brain/ physiology
  • Electric Stimulation
  • Electrophysiology
  • Evoked Potentials
  • Geniculate Bodies/physiology
  • Guinea Pigs
  • Perfusion
  • Superior Colliculi/physiology
  • Visual Cortex/physiology
  • Visual Pathways/physiology
Citation (ISO format)
MUHLETHALER, Michel et al. The isolated and perfused brain of the guinea-pig in vitro. In: European journal of neuroscience, 1993, vol. 5, n° 7, p. 915–926.
ISSN of the journal0953-816X

Technical informations

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