fr
Article scientifique
Anglais

Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain

Publié dansNature Methods, vol. 14, no. 5, p. 495-503
Date de publication2017
Résumé

Few tools exist to visualize and manipulate neurons that are targets of neuromodulators. We present iTango, a light- and ligand-gated gene expression system based on a light-inducible split tobacco etch virus protease. Cells expressing the iTango system exhibit increased expression of a marker gene in the presence of dopamine and blue-light exposure, both in vitro and in vivo. We demonstrated the iTango system in a behaviorally relevant context, by inducing expression of optogenetic tools in neurons under dopaminergic control during a behavior of interest. We thereby gained optogenetic control of these behaviorally relevant neurons. We applied the iTango system to decipher the roles of two classes of dopaminergic neurons in the mouse nucleus accumbens in a sensitized locomotor response to cocaine. Thus, the iTango platform allows for control of neuromodulatory circuits in a genetically and functionally defined manner with spatial and temporal precision.

Mots-clés
  • Animals
  • Behavior
  • Animal/physiology
  • Brain/cytology/metabolism
  • Brain Mapping/methods
  • Dopamine/metabolism/pharmacology
  • Endopeptidases/genetics
  • Gene Expression/drug effects/radiation effects
  • HEK293 Cells
  • Humans
  • Ligands
  • Light
  • Mice
  • Neural Pathways/physiology
  • Neurons/metabolism
  • Optogenetics/methods
  • Photic Stimulation
  • Rats
  • Receptors
  • Dopamine D2/genetics
  • Signal-To-Noise Ratio
Citation (format ISO)
LEE, Dongmin et al. Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. In: Nature Methods, 2017, vol. 14, n° 5, p. 495–503. doi: 10.1038/nmeth.4234
Fichiers principaux (1)
Article (Published version)
accessLevelRestricted
Identifiants
ISSN du journal1548-7091
496vues
6téléchargements

Informations techniques

Création14.12.2017 09:16:00
Première validation14.12.2017 09:16:00
Heure de mise à jour15.03.2023 07:41:39
Changement de statut15.03.2023 07:41:39
Dernière indexation17.01.2024 01:55:39
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack