Scientific Article
previous document  unige:100724  next document
add to browser collection
Title

Assessment of hepatic glucose metabolism by indirect calorimetry in combination with a non-invasive technique using naturally enriched 13C glucose in healthy children and adolescents

Authors
Selz, Roxane
Tappy, Luc
Woringer, Virgile
Theintz, Gérald E
Published in Hormone Research. 2004, vol. 62, no. 3, p. 142-8
Abstract The metabolic fate of hepatic glucose can be best studied using invasive techniques such as tracer infusions and frequent blood sampling which have been revealed to be impractical in the pediatric age group. The aim of this study was to develop a non-invasive method based on indirect calorimetry and expired 13CO2 monitoring in order to gain insight into the mechanisms leading to impaired glucose tolerance in children and teenagers. As a first step, net glucose oxidation (NGO) and energy expenditure (EE) were measured in 47 subjects (range 7.5-17.3 years) of whom 18 were prepubertal (P1), 11 in early puberty (P2-P3) and 18 in late puberty (P4-P5) after 3-hourly loads of 180 mg/kg of oral maize glucose containing naturally enriched 13C. Isotope analysis allowed to calculate exogenous and endogenous glucose oxidation (EXGO, ENGO) and, hence, to derive TGS and NGS, that is glycogen turnover. NGO and EE decreased significantly with pubertal progression, reflecting higher metabolism at younger ages, whereas EXGO remained constant. TGS did not change significantly whereas NGS showed a significant negative correlation with pubertal progression: this can be explained by the fact that glycogenolysis exceeded glycogen synthesis in this experimental setting. This non-invasive method appears to be a promising tool to study the fate of hepatic glucose and therefore glycogen turnover in children at risk of developing glucose intolerance and/or type 2 diabetes.
Keywords AdolescentCalorimetryIndirect/methods/standardsCarbon Dioxide/metabolismCarbon IsotopesChildFemaleGlucose/metabolismGlucose Intolerance/diagnosis/metabolismGlycogen/metabolismHumansLiver/metabolismMaleOxygen ConsumptionReference Values
Identifiers
PMID: 15297802
Full text
Article (Published version) (84 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Citation
(ISO format)
SELZ, Roxane et al. Assessment of hepatic glucose metabolism by indirect calorimetry in combination with a non-invasive technique using naturally enriched 13C glucose in healthy children and adolescents. In: Hormone Research, 2004, vol. 62, n° 3, p. 142-8. https://archive-ouverte.unige.ch/unige:100724

40 hits

0 download

Update

Deposited on : 2017-12-21

Export document
Format :
Citation style :