UNIGE document Scientific Article
previous document  unige:100723  next document
add to browser collection

Targeted expression of catalase to mitochondria prevents age-associated reductions in mitochondrial function and insulin resistance

Lee, Hui-Young
Choi, Cheol Soo
Birkenfeld, Andreas L
Alves, Tiago C
Jurczak, Michael J
Zhang, Dongyan
Woo, Dong Kyun
show hidden authors show all authors [1 - 15]
Published in Cell Metabolism. 2010, vol. 12, no. 6, p. 668-74
Abstract Aging-associated muscle insulin resistance has been hypothesized to be due to decreased mitochondrial function, secondary to cumulative free radical damage, leading to increased intramyocellular lipid content. To directly test this hypothesis, we examined both in vivo and in vitro mitochondrial function, intramyocellular lipid content, and insulin action in lean healthy mice with targeted overexpression of the human catalase gene to mitochondria (MCAT mice). Here, we show that MCAT mice are protected from age-induced decrease in muscle mitochondrial function (∼30%), energy metabolism (∼7%), and lipid-induced muscle insulin resistance. This protection from age-induced reduction in mitochondrial function was associated with reduced mitochondrial oxidative damage, preserved mitochondrial respiration and muscle ATP synthesis, and AMP-activated protein kinase-induced mitochondrial biogenesis. Taken together, these data suggest that the preserved mitochondrial function maintained by reducing mitochondrial oxidative damage may prevent age-associated whole-body energy imbalance and muscle insulin resistance.
Keywords Adenosine Triphosphate/biosynthesisAging/metabolismAnimalsCatalase/genetics/metabolismDNA DamageEnergy Metabolism/physiologyHumansInsulin/metabolismInsulin Resistance/physiologyMaleMiceMiceTransgenicMicroscopyElectronTransmissionMitochondria/metabolism/physiology/ultrastructureMuscleSkeletal/metabolismOxidative Stress/physiologyOxygen Consumption/physiologyReactive Oxygen Species/metabolism
PMID: 21109199
Full text
Article (Published version) (812 Kb) - document accessible for UNIGE members only Limited access to UNIGE
(ISO format)
LEE, Hui-Young et al. Targeted expression of catalase to mitochondria prevents age-associated reductions in mitochondrial function and insulin resistance. In: Cell Metabolism, 2010, vol. 12, n° 6, p. 668-74. doi: 10.1016/j.cmet.2010.11.004 https://archive-ouverte.unige.ch/unige:100723

265 hits

0 download


Deposited on : 2017-12-21

Export document
Format :
Citation style :