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EMMPRIN overexpression in SVZ neural progenitor cells increases their migration towards ischemic cortex

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Published in Experimental neurology. 2017, vol. 297, p. 14-24
Abstract Stimulation of endogenous neurogenesis and recruitment of neural progenitors from the subventricular zone (SVZ) neurogenic site may represent a useful strategy to improve regeneration in the ischemic cortex. Here, we tested whether transgenic overexpression of extracellular matrix metalloproteinase inducer (EMMPRIN), the regulator of matrix metalloproteinases (MMPs) expression, in endogenous neural progenitor cells (NPCs) in the subventricular zone (SVZ) could increase migration towards ischemic injury. For this purpose, we applied a lentivector-mediated gene transfer system. We found that EMMPRIN-transduced progenitors exhibited enhanced MMP-2 activity in vitro and showed improved motility in 3D collagen gel as well as in cortical slices. Using a rat model of neonatal ischemia, we showed that EMMPRIN overexpressing SVZ cells invade the injured cortical tissue more efficiently than controls. Our results suggest that EMMPRIN overexpression could be suitable approach to improve capacities of endogenous or transplanted progenitors to invade the injured cortex.
Keywords AnimalsNewbornBasigin/biosynthesis/geneticsBrain Ischemia/metabolism/pathologyCell Movement/physiologyCerebral Cortex/metabolism/pathologyGene ExpressionLateral Ventricles/metabolism/pathologyNeural Stem Cells/metabolismOrgan Culture TechniquesRatsSprague-DawleyWistar
PMID: 28716558
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Research groups Groupe Dayer Alexandre (Formation du circuit cortical) (875)
Progéniteurs neuronaux (141)
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KANEMITSU, Michiko et al. EMMPRIN overexpression in SVZ neural progenitor cells increases their migration towards ischemic cortex. In: Experimental Neurology, 2017, vol. 297, p. 14-24. doi: 10.1016/j.expneurol.2017.07.009 https://archive-ouverte.unige.ch/unige:100545

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Deposited on : 2017-12-19

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