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Scientific article
English

EMMPRIN overexpression in SVZ neural progenitor cells increases their migration towards ischemic cortex

Published inExperimental Neurology, vol. 297, p. 14-24
Publication date2017
Abstract

Stimulation of endogenous neurogenesis and recruitment of neural progenitors from the subventricular zone (SVZ) neurogenic site may represent a useful strategy to improve regeneration in the ischemic cortex. Here, we tested whether transgenic overexpression of extracellular matrix metalloproteinase inducer (EMMPRIN), the regulator of matrix metalloproteinases (MMPs) expression, in endogenous neural progenitor cells (NPCs) in the subventricular zone (SVZ) could increase migration towards ischemic injury. For this purpose, we applied a lentivector-mediated gene transfer system. We found that EMMPRIN-transduced progenitors exhibited enhanced MMP-2 activity in vitro and showed improved motility in 3D collagen gel as well as in cortical slices. Using a rat model of neonatal ischemia, we showed that EMMPRIN overexpressing SVZ cells invade the injured cortical tissue more efficiently than controls. Our results suggest that EMMPRIN overexpression could be suitable approach to improve capacities of endogenous or transplanted progenitors to invade the injured cortex.

Keywords
  • Animals
  • Newborn
  • Basigin/biosynthesis/genetics
  • Brain Ischemia/metabolism/pathology
  • Cell Movement/physiology
  • Cerebral Cortex/metabolism/pathology
  • Gene Expression
  • Lateral Ventricles/metabolism/pathology
  • Neural Stem Cells/metabolism
  • Organ Culture Techniques
  • Rats
  • Sprague-Dawley
  • Wistar
Citation (ISO format)
KANEMITSU, Michiko et al. EMMPRIN overexpression in SVZ neural progenitor cells increases their migration towards ischemic cortex. In: Experimental Neurology, 2017, vol. 297, p. 14–24. doi: 10.1016/j.expneurol.2017.07.009
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Article (Published version)
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Identifiers
ISSN of the journal0014-4886
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