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Scientific article
English

Deletion of the mammalian INDY homolog mimics aspects of dietary restriction and protects against adiposity and insulin resistance in mice

Published inCell Metabolism, vol. 14, no. 2, p. 184-195
Publication date2011
Abstract

Reduced expression of the Indy (I'm Not Dead, Yet) gene in D. melanogaster and its homolog in C. elegans prolongs life span and in D. melanogaster augments mitochondrial biogenesis in a manner akin to caloric restriction. However, the cellular mechanism by which Indy does this is unknown. Here, we report on the knockout mouse model of the mammalian Indy (mIndy) homolog, SLC13A5. Deletion of mIndy in mice (mINDY(-/-) mice) reduces hepatocellular ATP/ADP ratio, activates hepatic AMPK, induces PGC-1α, inhibits ACC-2, and reduces SREBP-1c levels. This signaling network promotes hepatic mitochondrial biogenesis, lipid oxidation, and energy expenditure and attenuates hepatic de novo lipogenesis. Together, these traits protect mINDY(-/-) mice from the adiposity and insulin resistance that evolve with high-fat feeding and aging. Our studies demonstrate a profound effect of mIndy on mammalian energy metabolism and suggest that mINDY might be a therapeutic target for the treatment of obesity and type 2 diabetes.

Keywords
  • Adiposity/genetics
  • Aging
  • Animals
  • Caloric Restriction
  • Dicarboxylic Acid Transporters
  • Energy Metabolism/genetics
  • HEK293 Cells
  • Humans
  • Insulin Resistance/genetics
  • Lipid Metabolism/genetics
  • Mice
  • Mice
  • Knockout
  • Mitochondria/genetics/metabolism
  • Obesity/genetics
  • Symporters/biosynthesis/deficiency/genetics
Affiliation Not a UNIGE publication
Citation (ISO format)
BIRKENFELD, Andreas L et al. Deletion of the mammalian INDY homolog mimics aspects of dietary restriction and protects against adiposity and insulin resistance in mice. In: Cell Metabolism, 2011, vol. 14, n° 2, p. 184–195. doi: 10.1016/j.cmet.2011.06.009
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Article (Published version)
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Identifiers
ISSN of the journal1550-4131
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