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Dual function of NRP1 in axon guidance and subcellular target recognition in cerebellum

Cioni, Jean-Michel
Saywell, Veronique
Jahannault-Talignani, Céline
Huettl, Rosa E
Sarrailh-Faivre, Catherine
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Published in Neuron. 2016, vol. 91, no. 6, p. 1276-1291
Abstract Subcellular target recognition in the CNS is the culmination of a multiple-step program including axon guidance, target recognition, and synaptogenesis. In cerebellum, basket cells (BCs) innervate the soma and axon initial segment (AIS) of Purkinje cells (PCs) to form the pinceau synapse, but the underlying mechanisms remain incompletely understood. Here, we demonstrate that neuropilin-1 (NRP1), a Semaphorin receptor expressed in BCs, controls both axonal guidance and subcellular target recognition. We show that loss of Semaphorin 3A function or specific deletion of NRP1 in BCs alters the stereotyped organization of BC axon and impairs pinceau synapse formation. Further, we identified NRP1 as a trans-synaptic binding partner of the cell adhesion molecule neurofascin-186 (NF186) expressed in the PC AIS during pinceau synapse formation. These findings identify a dual function of NRP1 in both axon guidance and subcellular target recognition in the construction of GABAergic circuitry.
Keywords AnimalsAxon Guidance/physiologyCHO CellsCell Adhesion Molecules/metabolismCerebellum/cytology/growth & developmentCoculture TechniquesCricetulusGABAergic Neurons/physiologyHumansNerve Growth Factors/metabolismNeurogenesis/physiologyNeuropilin-1/physiologyPurkinje Cells/physiologySemaphorin-3A/physiologySynapses/physiology
PMID: 27618676
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Research group Groupe Dayer Alexandre (Formation du circuit cortical) (875)
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TELLEY, Ludovic et al. Dual function of NRP1 in axon guidance and subcellular target recognition in cerebellum. In: Neuron, 2016, vol. 91, n° 6, p. 1276-1291. doi: 10.1016/j.neuron.2016.08.015 https://archive-ouverte.unige.ch/unige:100311

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Deposited on : 2017-12-14

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