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Title

A new real-time RT-PCR targeting VP4-VP2 to detect and quantify enterovirus D68 in respiratory samples

Authors
Josset, Laurence
Schuffenecker, Isabelle
Published in Journal of Medical Virology. 2017, vol. 89, no. 10, p. 1879-1881
Abstract Causing an international outbreak of respiratory disease, Enterovirus D68 quickly entered the closed circle of emerging viral pathogens of public health significance. As rapid and accurate detection of EV-D68 is essential for an efficient clinical management, we designed and validated a new highly efficient one-step quantitative rRT-PCR specific to EV-D68 VP4-VP2 region. With 100% specificity and 95.6% sensitivity to all EV-D68 strains, this new assay can be reliably used to detect and quantify EV-D68 in respiratory samples and represents an interesting additional tool for diagnosis as it targets an original region of the genome.
Identifiers
PMID: 28169437
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Article (Published version) (157 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Identification des facteurs clés impliqués dans la pathogénèse des rhinovirus et entérovirus (953)
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(ISO format)
ROYSTON, Lena et al. A new real-time RT-PCR targeting VP4-VP2 to detect and quantify enterovirus D68 in respiratory samples. In: Journal of Medical Virology, 2017, vol. 89, n° 10, p. 1879-1881. https://archive-ouverte.unige.ch/unige:100257

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Deposited on : 2017-12-13

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