UNIGE document Scientific Article
previous document  unige:100164  next document
add to browser collection

Cux1 Enables Interhemispheric Connections of Layer II/III Neurons by Regulating Kv1-Dependent Firing

Rodríguez-Tornos, Fernanda M
Briz, Carlos G
Weiss, Linnea A
Sebastián-Serrano, Alvaro
Ares, Saúl
Navarrete, Marta
Galazo, Maria
show hidden authors show all authors [1 - 11]
Published in Neuron. 2016, vol. 89, no. 3, p. 494-506
Abstract Neuronal subtype-specific transcription factors (TFs) instruct key features of neuronal function and connectivity. Activity-dependent mechanisms also contribute to wiring and circuit assembly, but whether and how they relate to TF-directed neuronal differentiation is poorly investigated. Here we demonstrate that the TF Cux1 controls the formation of the layer II/III corpus callosum (CC) projections through the developmental transcriptional regulation of Kv1 voltage-dependent potassium channels and the resulting postnatal switch to a Kv1-dependent firing mode. Loss of Cux1 function led to a decrease in the expression of Kv1 transcripts, aberrant firing responses, and selective loss of CC contralateral innervation. Firing and innervation were rescued by re-expression of Kv1 or postnatal reactivation of Cux1. Knocking down Kv1 mimicked Cux1-mediated CC axonal loss. These findings reveal that activity-dependent processes are central bona fide components of neuronal TF-differentiation programs and establish the importance of intrinsic firing modes in circuit assembly within the neocortex.
Keywords Action Potentials/physiologyAnimalsCorpus Callosum/cytology/growth & development/physiologyGene Expression Regulation, DevelopmentalGene Knockdown TechniquesHomeodomain Proteins/metabolismMiceMice, TransgenicNeurons/physiologyNuclear Proteins/metabolismPrimary Cell CultureRepressor Proteins/metabolismShaker Superfamily of Potassium Channels/biosynthesis/genetics/physiology
PMID: 26804994
Full text
Article (Published version) (6 MB) - public document Free access
Research group Neurobiologie développementale et plasticité (880)
(ISO format)
RODRÍGUEZ-TORNOS, Fernanda M et al. Cux1 Enables Interhemispheric Connections of Layer II/III Neurons by Regulating Kv1-Dependent Firing. In: Neuron, 2016, vol. 89, n° 3, p. 494-506. doi: 10.1016/j.neuron.2015.12.020 https://archive-ouverte.unige.ch/unige:100164

408 hits



Deposited on : 2017-12-12

Export document
Format :
Citation style :