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Title

Cux1 Enables Interhemispheric Connections of Layer II/III Neurons by Regulating Kv1-Dependent Firing

Authors
Rodríguez-Tornos, Fernanda M
Briz, Carlos G
Weiss, Linnea A
Sebastián-Serrano, Alvaro
Ares, Saúl
Navarrete, Marta
Galazo, Maria
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Published in Neuron. 2016, vol. 89, no. 3, p. 494-506
Abstract Neuronal subtype-specific transcription factors (TFs) instruct key features of neuronal function and connectivity. Activity-dependent mechanisms also contribute to wiring and circuit assembly, but whether and how they relate to TF-directed neuronal differentiation is poorly investigated. Here we demonstrate that the TF Cux1 controls the formation of the layer II/III corpus callosum (CC) projections through the developmental transcriptional regulation of Kv1 voltage-dependent potassium channels and the resulting postnatal switch to a Kv1-dependent firing mode. Loss of Cux1 function led to a decrease in the expression of Kv1 transcripts, aberrant firing responses, and selective loss of CC contralateral innervation. Firing and innervation were rescued by re-expression of Kv1 or postnatal reactivation of Cux1. Knocking down Kv1 mimicked Cux1-mediated CC axonal loss. These findings reveal that activity-dependent processes are central bona fide components of neuronal TF-differentiation programs and establish the importance of intrinsic firing modes in circuit assembly within the neocortex.
Keywords Action Potentials/physiologyAnimalsCorpus Callosum/cytology/growth & development/physiologyGene Expression Regulation, DevelopmentalGene Knockdown TechniquesHomeodomain Proteins/metabolismMiceMice, TransgenicNeurons/physiologyNuclear Proteins/metabolismPrimary Cell CultureRepressor Proteins/metabolismShaker Superfamily of Potassium Channels/biosynthesis/genetics/physiology
Identifiers
PMID: 26804994
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Article (Published version) (6 MB) - document accessible for UNIGE members only Limited access to UNIGE
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Research group Neurobiologie développementale et plasticité (880)
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RODRÍGUEZ-TORNOS, Fernanda M et al. Cux1 Enables Interhemispheric Connections of Layer II/III Neurons by Regulating Kv1-Dependent Firing. In: Neuron, 2016, vol. 89, n° 3, p. 494-506. https://archive-ouverte.unige.ch/unige:100164

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Deposited on : 2017-12-12

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