Sendai virus C proteins counteract the interferon-mediated induction of an antiviral state

Article Article scientifique Article Sendai virus C proteins counteract the interferon-mediated induction of an antiviral state eng 1999 author Garcin Dominique 24296 author Latorre Patrizia 99162 author Kolakofsky Daniel 25420 6559-6565 10.1128/JVI.73.8.6559-6565.1999 10400752 0022-538X We have studied the relationship between the Sendai virus (SeV) C proteins (a nested set of four proteins initiated at different start codons) and the interferon (IFN)-mediated antiviral response in IFN-competent cells in culture. SeV strains containing wild-type or various mutant C proteins were examined for their ability (i) to induce an antiviral state (i.e., to prevent the growth of vesicular stomatitis virus [VSV] following a period of SeV infection), (ii) to induce the elevation of Stat1 protein levels, and (iii) to prevent IFN added concomitant with the SeV infection from inducing an antiviral state. We find that expression of the wild-type C gene and, specifically, the AUG114-initiated C protein prevents the establishment of an antiviral state: i.e., cells infected with wild-type SeV exhibited little or no increase in Stat1 levels and were permissive for VSV replication, even in the presence of exogenous IFN. In contrast, in cells infected with SeV lacking the AUG114-initiated C protein or containing a single amino acid substitution in the C protein, the level of Stat1 increased and VSV replication was inhibited. The prevention of the cellular IFN-mediated antiviral response appears to be a key determinant of SeV pathogenicity. Animals Antiviral Agents/metabolism/pharmacology Binding Sites Cell Line Cricetinae DNA-Binding Proteins/metabolism Interferon-beta/metabolism/pharmacology Macaca mulatta Mice Phosphoproteins Recombinant Proteins/metabolism/pharmacology Respirovirus/genetics/metabolism/physiology STAT1 Transcription Factor Trans-Activators/metabolism Vesicular stomatitis Indiana virus/growth & development/metabolism Viral Proteins/genetics/physiology Viral Structural Proteins/metabolism Dewey 616 54 Département de microbiologie et médecine moléculaire 21B false 216 Virologie moléculaire 275 Journal of virology 73 8 590448ef-c978-4b11-8422-aa7a8884d2a3 Article (Published version) Public jv006559.pdf 1661248 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Sendai virus C proteins counteract the interferon-mediated induction of an antiviral state	Journal of virology			1999	654	387

Archive ouverte UNIGE

Latorre, Patrizia