Distinct parasegmental and imaginal enhancers and the establishment of the expression pattern of the Ubx gene

Article Article scientifique Article Distinct parasegmental and imaginal enhancers and the establishment of the expression pattern of the Ubx gene eng 1995 author Pirrotta Vincenzo 28459 author Chan Chi Shing author Mccabe Pirrotta Donna 99160 author Qian Su 1439-1450 10.1093/genetics/141.4.1439 8601485 0016-6731 The expression domain of the Ubx gene in Drosophila embryos is bounded by the product of the hb gene, acting as a repressor. We show that all Ubx fragments that bind Hb protein in vitro contain parasegmental enhancers active in the embryo in specific parasegmental patterns. We have found three new embryonic enhancer elements in the upstream region, in addition to the two previously identified. Each produces a pattern initially bounded at PS6 by Hb but sooner or later breaks down this boundary and begins to express in the anterior region. These enhancers do not respond to the long-term maintenance mediated by the Polycomb group of genes. They also cease functioning after germ band extension. Expression in imaginal tissues is due to a set of entirely separate and independent imaginal disc enhancers. These do not contain Hb binding sites and by themselves have no anterior/posterior positional information, although some distinguish between ventral and dorsal discs. A third kind of element, the Polycomb Response Element (PRE), has no enhancer activity but causes long-term maintenance of the expression domain of other enhancers present in the vicinity. The interaction of these elements results in the correct expression of Ubx in imaginal tissues. Animals Bacterial Proteins/metabolism Base Sequence Binding Sites DNA DNA Footprinting DNA-Binding Proteins/genetics/metabolism Drosophila/embryology/genetics Drosophila Proteins Enhancer Elements Genetic Gene Expression Regulation Developmental Homeodomain Proteins/genetics Molecular Sequence Data Regulatory Sequences Nucleic Acid Transcription Factors Dewey 590 44 Département de génétique et évolution 162 false Genetics 141 4 77053135-9eb9-47f7-80c5-9c4977dd6d14 Article (Published version) Restricted V.Pirrotta_3.pdf 11630718 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

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Distinct parasegmental and imaginal enhancers and the establishment of the expression pattern of the Ubx gene	Genetics			1995	329	1

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Mccabe Pirrotta, Donna