Investigating the cutaneous delivery and spatiotemporal biodistribution of highly lipophilic natural products from nanocarrier systems

Diplôme Thèse Investigating the cutaneous delivery and spatiotemporal biodistribution of highly lipophilic natural products from nanocarrier systems eng 2026-02-06 2026-02-06 author Syafitri Erga 984233 director Kalia Yogeshvar 0000-0001-9049-5489 503798 Section des sciences pharmaceutiques (FARMA) 187 10.13097/archive-ouverte/unige:192322 urn:nbn:ch:unige-1923226 Sc. Vie - Pharma. 381 The topical delivery of plant-derived natural products is often limited by their high lipophilicity, poor aqueous solubility, and formulation instability. This thesis aimed to develop aqueous nanocarrier formulations and investigate their ability to enhance the cutaneous delivery of highly lipophilic natural products from both single compounds and complex plant mixtures. Cutaneous biodistribution was investigated using the cutaneous biodistribution method (CBM), combining cryosectioning with UHPLC-MS/MS to enable depth-resolved monitoring of single and multiple lipophilic compounds. Among the nanocarrier systems evaluated, nanoemulsions outperformed micelles and microemulsions, improving delivery to the viable epidermis and dermis, with evidence of preferential deposition in hair follicles. Importantly, this work also investigated biodistribution over extended periods using a viable ex vivo skin culture model to better represent real-life topical application, revealing progressive accumulation of bioactive compounds in the epidermis and upper dermis while maintaining tissue integrity. These findings demonstrate the potential of nanoemulsion-based formulations and advanced biodistribution analysis for improving the topical delivery of lipophilic natural products. Cutaneous delivery Lipophilic natural products Skin biodistribution Ex vivo skin culture model Nanocarriers Dewey 615 31 Section des sciences pharmaceutiques 17 false 808bfa4f-ba6c-4d26-be5e-408ab221affc Thesis Restricted Erga_Syafitri_Thesis_Final_Version.pdf 6339875 application/pdf Private 2027-02-06 78a6636ab9375bf4ee7228c71c0f5543965dd8cf296c4fa1de992a89144cfd45 33c530c8-9d0c-42f9-9421-4e91cb80ea1c Imprimatur Public Imprimatur.pdf 64236 application/pdf dc2b90c7c1aa21be480aa0559c51763cae7c75a68296e7814f19c24db803eb59 embargoedAccess Faculté des sciences / Section des sciences pharmaceutiques

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