Cytokinetic abscission in <i>Toxoplasma gondii</i> is governed by protein phosphatase 2A and the daughter cell scaffold complex

Article Article scientifique Article Cytokinetic abscission in <i>Toxoplasma gondii</i> is governed by protein phosphatase 2A and the daughter cell scaffold complex eng 2024-07-15 author Marq Jean-Baptiste 27225 Département de microbiologie et médecine moléculaire (MIMOL) author Gosetto Margaux 895980 author Altenried Aline 983957 author Vadas Oscar 0000-0003-3511-6479 508966 Département de microbiologie et médecine moléculaire (MIMOL) author Maco Bohumil 0000-0001-8444-313X 863802 Services communs de la faculté de médecine (SCMU) author Dos Santos Pacheco Nicolas 0000-0003-1959-194X 880386 author Tosetti Nicolo 828676 author Soldati-Favre Dominique 0000-0003-4156-2109 135114 Département de microbiologie et médecine moléculaire (MIMOL) author Lentini Gaëlle 883368 10.1038/s44318-024-00171-9 39009675 0261-4189 https://www.embopress.org/doi/full/10.1038/s44318-024-00171-9 Cytokinetic abscission marks the final stage of cell division, during which the daughter cells physically separate through the generation of new barriers, such as the plasma membrane or cell wall. While the contractile ring plays a central role during cytokinesis in bacteria, fungi and animal cells, the process diverges in Apicomplexa. In Toxoplasma gondii , two daughter cells are formed within the mother cell by endodyogeny. The mechanism by which the progeny cells acquire their plasma membrane during the disassembly of the mother cell, allowing daughter cells to emerge, remains unknown. Here we identify and characterize five T. gondii proteins, including three protein phosphatase 2A subunits, which exhibit a distinct and dynamic localization pattern during parasite division. Individual downregulation of these proteins prevents the accumulation of plasma membrane at the division plane, preventing the completion of cellular abscission. Remarkably, the absence of cytokinetic abscission does not hinder the completion of subsequent division cycles. The resulting progeny are able to egress from the infected cells but fail to glide and invade, except in cases of conjoined twin parasites. Abscission Cytokinesis Cytoskeleton Division Plane Phosphatase Dewey 616 Swiss National Science Foundation An integrated approach to elucidate the molecular mechanisms governing host cell invasion and egress by the Apicomplexa 185325 CH Projects Swiss National Science Foundation An integrated approach to elucidate the molecular mechanisms governing host cell invasion and egress by the Apicomplexa 215445 CH Projects European Commission Molecular Basis of Toxoplasma gondii Encystation and Persistence 695596 ToxoPersist EU H2020 54 Département de microbiologie et médecine moléculaire 21B false 443 Biologie d'un parasite intracellulaire obligatoire 773 https://www.ebi.ac.uk/pride/archive/projects/PXD051495 https://www.ebi.ac.uk/pride/archive/projects/PXD047907 https://www.ebi.ac.uk/biostudies/sourcedata/studies/S-SCDT-10_1038-S44318-024-00171-9 EMBO journal 7c226556-5295-435a-86f7-fa93e7fc7d36 Article (Published version) Public marq-et-al-2024-cytokinetic-absciss.pdf 15848202 application/pdf CC BY-4.0 0bd9e8de1171c1d432dfcead76c8b2d9e29070672ae246144ae89b6b1eb5f1e1 e0b2329b-3cfd-4115-8d97-6c21f0a60b9f Supplemental data Public Table_EV1.xlsx 21396 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet f6da3995fa6bfb0a8b8fdf0b374c5c23bb6e07c0a2bb7e4689edca86f50c35a6 c9a6e116-32be-440d-a36e-2152df08ada9 Supplemental data Public Table_EV2.xlsx 21515 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 1281894bdd14d9c9cf3c738164120e5761e276eb3cea1ab111ba60702a26b3f1 0043ff3b-b22a-4364-9148-235b7ecf74c5 Supplemental data Public Table_EV3.xlsx 20259 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 647134e25dc8940acf49d4621a29f68a55318d4c2750d148c9b8fd7bc5028e04 c2efca42-14fc-4817-9648-0a16d4b4aeb1 Supplemental data Public APPENDIX.pdf 955643 application/pdf 61942e80419d2c609b12e06871871019842ac776219135544e964396ade9896d a28a02c2-f4d2-4422-bc11-3bcc56e791c7 Supplemental data Public DATA_SET_EV1.xlsx 358321 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet d7decd8b334b5ff5fe62460c3776d36b9b949490b84a8d813a22dfecb20cf08f 034dcf52-ea8d-4aea-86fc-b5fc191bde41 Supplemental data Public DATA_SET_EV2.xlsx 141170 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 3711ffecb82fa608259f8f51270efc624bf44659f19e40fc507402680f43fa1b cf8210b9-ef85-47c9-a6e8-e67c7e9e2238 Supplemental data Public DATA_SET_EV3.xlsx 203866 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 7d1ceaf252d5293ba15cc39535faaf81fb168b1b565a87c3e555c1fc28042284 f81d93ca-ba7b-469b-994b-6a9bee59456c Supplemental data Public DATA_SET_EV4.xlsx 28695 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 51e49a40b5d3738f10dfa676ab703e83c417baded74e07e213d04bd6131ac24f 13a95192-32f3-4e49-96aa-5a8aa2e6ce27 Supplemental data Public Expanded_View_Figures.pdf 4445169 application/pdf a03f187a1a200a9878034c09a86abb762061fe0c8da4649588691720a79de002 Vignette Public 44318_2024_171_figa_html.jpg 335339 image/png openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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Cytokinetic abscission in Toxoplasma gondii is governed by protein phosphatase 2A and the daughter cell scaffold complex	EMBO journal			2024	154	94

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