Jump ahead with a twist: DNA acrobatics drive transposition forward

Article Article scientifique Revue de la littérature Jump ahead with a twist: DNA acrobatics drive transposition forward eng 2019-12 author Arinkin Vladimir 0000-0002-9210-9366 980415 Département de biologie moléculaire et cellulaire (MOCEL) author Smyshlyaev Georgy 951631 author Barabas Orsolya 938832 Département de biologie moléculaire et cellulaire (MOCEL) 168-177 10.1016/j.sbi.2019.08.006 31590109 0959-440X PMC6900584 https://linkinghub.elsevier.com/retrieve/pii/S0959440X18302161 <p>Transposases move discrete pieces of DNA between genomic locations and had a profound impact on evolution. They drove the emergence of important biological functions and are the most frequent proteins encoded in modern genomes. Yet, the molecular principles of their actions have remained largely unclear. Here we review recent structural studies of transposase-DNA complexes and related cellular machineries, which provided unmatched mechanistic insights. We highlight how transposases introduce major DNA twists and kinks at various stages of their reaction and discuss the functional impact of these astounding DNA acrobatics on several aspects of transposition. By comparison with distantly related DNA recombination systems, we propose that forcing DNA into unnatural shapes may be a general strategy to drive rearrangements forward.</p> Deutsche Forschungsgemeinschaft BA 5276/2-1 NIGMS NIH HHS P41 GM103311 N.I.H. P41-GM103311 EMBL Federal Ministry of Education and Research 01KI1706 true Current opinion in structural biology 59 da90fd64-9aaf-429c-bbd4-2146e8b12e27 Article (Published version) Public BARABAS01.pdf 3358421 application/pdf CC BY-4.0 145fe2f2524c7c8bb9dd8c353c27c4b9610d9539ec6d280626c685f57d720da2 openAccess

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Jump ahead with a twist: DNA acrobatics drive transposition forward	Current opinion in structural biology			2019	33	10

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Arinkin, Vladimir