Structural and regulatory insights into the glideosome-associated connector from Toxoplasma gondii

Article Article scientifique Article Structural and regulatory insights into the glideosome-associated connector from Toxoplasma gondii eng 2023-04-04 2023-04-04 author Kumar Amit author Vadas Oscar 0000-0003-3511-6479 508966 Département de microbiologie et médecine moléculaire (MIMOL) author Dos Santos Pacheco Nicolas 0000-0003-1959-194X 880386 Département de microbiologie et médecine moléculaire (MIMOL) author Zhang Xu author Chao Kin author Darvill Nicolas author Rasmussen Helena Ø 0000-0001-8384-656X author Xu Yingqi author Lin Gloria 979975 Département de microbiologie et médecine moléculaire (MIMOL) author Stylianou Fisentzos A author Pedersen Jan Skov author Rouse Sarah L author Morgan Marc L author Soldati-Favre Dominique 0000-0003-4156-2109 135114 Département de microbiologie et médecine moléculaire (MIMOL) author Matthews Stephen 0000-0003-0676-0927 e86049 10.7554/eLife.86049 37014051 2050-084X https://elifesciences.org/articles/86049 <p> The phylum of Apicomplexa groups intracellular parasites that employ substrate-dependent gliding motility to invade host cells, egress from the infected cells and cross biological barriers. The glideosome associated connector (GAC) is a conserved protein essential to this process. GAC facilitates the association of actin filaments with surface transmembrane adhesins and the efficient transmission of the force generated by myosin translocation of actin to the cell surface substrate. Here, we present the crystal structure of Toxoplasma gondii GAC and reveal a unique, supercoiled armadillo repeat region that adopts a closed ring conformation. Characterisation of the solution properties together with membrane and F-actin binding interfaces suggest that GAC adopts several conformations from closed to open and extended. A multi-conformational model for assembly and regulation of GAC within the glideosome is proposed. </p> P. falciparum Human Infectious disease Microbiology Molecular biophysics Structural biology Dewey 616 Swiss National Science Foundation Structure-Function Relationship of the Guanylate Cyclase Signaling Platform in Apicomplexa 198545 CH Programmes Swiss National Science Foundation An integrated approach to elucidate the molecular mechanisms governing host cell invasion and egress by the Apicomplexa 185325 CH Projects 54 Département de microbiologie et médecine moléculaire 21B false 443 Biologie d'un parasite intracellulaire obligatoire 773 eLife 12 ea43a840-a4c0-4041-b06c-94016e29104c Article (Accepted version) Public Article-scientifique-from-crossref.pdf 43706182 application/pdf CC BY d53f97d60c772ce7f72b439e4a3ab5150855730898011100e04caf54d14cab02 openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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Structural and regulatory insights into the glideosome-associated connector from Toxoplasma gondii	eLife			2023	226	122

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