Vps60 initiates alternative ESCRT-III filaments

Article Article scientifique Article Vps60 initiates alternative ESCRT-III filaments eng 2023-09-28 2023-11-06 author Pfitzner Anna-Katharina 0000-0001-9952-5525 865904 author Zivkovic Henry 0009-0006-1410-7177 921462 author Bernat Cesar 0000-0002-7455-2049 979062 Département de biochimie (CHIBI) author West Matt 0000-0002-4710-9252 author Peltier Tanner 0000-0002-8750-2956 author Humbert Frédéric Jean-Christian 0009-0005-5265-7842 730215 Département de biochimie (CHIBI) author Odorizzi Greg 0000-0002-1143-1098 author Roux Aurélien 0000-0002-6088-0711 716946 Département de biochimie (CHIBI) e202206028 10.1083/jcb.202206028 37768378 0021-9525 PMC10538557 https://rupress.org/jcb/article/222/11/e202206028/276304/Vps60-initiates-alternative-ESCRT-III <p>Endosomal sorting complex required for transport-III (ESCRT-III) participates in essential cellular functions, from cell division to endosome maturation. The remarkable increase of its subunit diversity through evolution may have enabled the acquisition of novel functions. Here, we characterize a novel ESCRT-III copolymer initiated by Vps60. Membrane-bound Vps60 polymers recruit Vps2, Vps24, Did2, and Ist1, as previously shown for Snf7. Snf7- and Vps60-based filaments can coexist on membranes without interacting as their polymerization and recruitment of downstream subunits remain spatially and biochemically separated. In fibroblasts, Vps60/CHMP5 and Snf7/CHMP4 are both recruited during endosomal functions and cytokinesis, but their localization is segregated and their recruitment dynamics are different. Contrary to Snf7/CHMP4, Vps60/CHMP5 is not recruited during nuclear envelope reformation. Taken together, our results show that Vps60 and Snf7 form functionally distinct ESCRT-III polymers, supporting the notion that diversification of ESCRT-III subunits through evolution is linked to the acquisition of new cellular functions.</p> Dewey 540 National Institutes of Health NIGMS NIH HHS GM111335 NIH HHS FundaciÃ³n Alfonso MartÃn Escudero European Research Council 951324 National Institute of General Medical Sciences T32GZM142607 Swiss National Science Foundation MEETICS: MEmbrane Extension Transduces Information between Cellular Scales 200793 CH Projects 39 Département de biochimie 144 false 769 Groupe Roux The Journal of cell biology 222 11 cd0ff6ba-8513-4012-b018-acdd5092d20e Article (Published version) Public Article-scientifique-from-unpaywall.pdf 11671076 application/pdf CC BY 3fb21fe84bbffb311260db2b5b1f1e311b8d67e8e1113a57ef7588bdd696a8bd openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie

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Vps60 initiates alternative ESCRT-III filaments	The Journal of cell biology			2023	149	100

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Bernat, Cesar