Temperature-dependent polar lignification of a seed coat suberin layer promoting dormancy in Arabidopsis thaliana

Article Article scientifique Article Temperature-dependent polar lignification of a seed coat suberin layer promoting dormancy in Arabidopsis thaliana eng 2025-02-07 2025-02-11 author Hyvärinen Lena 0009-0007-9498-4846 Department of Plant Sciences, University of Geneva, Geneva 1211, Switzerland. 805118 author Fuchs Christelle 0000-0001-8181-2294 Department of Plant Sciences, University of Geneva, Geneva 1211, Switzerland. 823460 Département des sciences végétales (BIVEG) author Utz-Pugin Anne Department of Plant Sciences, University of Geneva, Geneva 1211, Switzerland. 22561 author Gully Kay Department of Plant Molecular Biology, University of Lausanne, Lausanne 1015, Switzerland. author Megies Christian Department of Plant Sciences, University of Geneva, Geneva 1211, Switzerland. 653026 Département des sciences végétales (BIVEG) author Holbein Julia 0000-0001-5146-9907 Department of Ecophysiology, Institute of Cellular and Molecular Botany, University of Bonn, Bonn 53115, Germany. author Iwasaki Mayumi 0000-0001-6561-4247 Department of Plant Sciences, University of Geneva, Geneva 1211, Switzerland. 728942 Département des sciences végétales (BIVEG) author Demonsais Lara Department of Plant Sciences, University of Geneva, Geneva 1211, Switzerland. author Capitao Maria Beatriz 0009-0003-8102-7087 Department of Plant Sciences, University of Geneva, Geneva 1211, Switzerland. 974771 Département des sciences végétales (BIVEG) author Barberon Marie 0000-0002-8169-8580 Department of Plant Sciences, University of Geneva, Geneva 1211, Switzerland. 898432 Département des sciences végétales (BIVEG) author Franke Rochus B 0000-0003-2269-7390 Department of Ecophysiology, Institute of Cellular and Molecular Botany, University of Bonn, Bonn 53115, Germany. author Nawrath Christiane 0000-0003-1470-8459 Department of Plant Molecular Biology, University of Lausanne, Lausanne 1015, Switzerland. author Loubery Sylvain Philippe 0000-0001-7061-9456 Department of Plant Sciences, University of Geneva, Geneva 1211, Switzerland. 669207 Département des sciences végétales (BIVEG) author Lopez Molina Luis 0000-0003-0463-1187 Department of Plant Sciences, University of Geneva, Geneva 1211, Switzerland.,Institute of Genetics and Genomics in Geneva, University of Geneva, Geneva 1211, Switzerland. 28870 Département des sciences végétales (BIVEG) e2413627122 10.1073/pnas.2413627122 39918953 0027-8424 https://pnas.org/doi/10.1073/pnas.2413627122 The seed is a landmark plant adaptation where the embryo is sheltered by a protective seed coat to facilitate dispersion. In Arabidopsis , the seed coat, derived from ovular integuments, plays a critical role in maintaining dormancy, ensuring germination occurs during a favorable season. Dormancy is enhanced by cold temperatures during seed development by affecting seed coat permeability through changes in apoplastic barriers. However, their localization and composition are poorly understood. This study identifies and investigates a polar barrier in the seed coat's outer integument (oi1) cells. We present histological, biochemical, and genetic evidence showing that cold promotes polar seed coat lignification of the outer integument 1 (oi1) cells and suberization throughout the entire oi1 cell boundary. The polar oi1 barrier is regulated by the transcription factors MYB107 and MYB9. MYB107, in particular, is crucial for the lignified polar oi1 barrier formation under cold temperatures. The absence of the oi1 barrier in mutant seeds correlates with increased permeability and reduced dormancy. Our findings elucidate how temperature-induced modifications in seed coat composition regulate dormancy, highlighting the roles of suberin and lignin in this process. Apoplastic barriers Lignin Seed coat Seed dormancy Suberin Dewey 580 Swiss National Science Foundation Investigating cold-induced modifications of seed coat apoplastic barriers and their role for the control of seed dormancy in Arabidopsis thaliana 219559 CH Projects 45 Département des sciences végétales 163 false 831 Groupe Lopez-Molina Proceedings of the National Academy of Sciences of the United States of America 122 6 e09f8da6-d4ef-4bcd-8ec6-a9d0117ccee8 Article (Published version) Public hyvarinen-et-al-2025.pdf 5444289 application/pdf CC BY-4.0 25c1e41f707d7401aa26b50ec53b6c282bcd78cded6ce54c88692dfe01338c7f openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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Temperature-dependent polar lignification of a seed coat suberin layer promoting dormancy in Arabidopsis thaliana	Proceedings of the National Academy of Sciences of the United States of America			2025	71	153

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