An In-depth Guide to the Ultrastructural Expansion Microscopy (U-ExM) of Chlamydomonas reinhardtii

Article Article scientifique Article An In-depth Guide to the Ultrastructural Expansion Microscopy (U-ExM) of Chlamydomonas reinhardtii eng 2023 author Klena Nikolai 868290 author Maltinti Giovanni author Batman Umut 974177 Département de biologie moléculaire et cellulaire (MOCEL) author Pigino Gaia author Guichard Paul 845268 Département de biologie moléculaire et cellulaire (MOCEL) author Hamel Virginie 846734 Département de biologie moléculaire et cellulaire (MOCEL) 10.21769/BioProtoc.4792 37719077 2331-8325 PMC10502176 https://bio-protocol.org/en/bpdetail?id=4792&type=0 Expansion microscopy is an innovative method that enables super-resolution imaging of biological materials using a simple confocal microscope. The principle of this method relies on the physical isotropic expansion of a biological specimen cross-linked to a swellable polymer, stained with antibodies, and imaged. Since its first development, several improved versions of expansion microscopy and adaptations for different types of samples have been produced. Here, we show the application of ultrastructure expansion microscopy (U-ExM) to investigate the 3D organization of the green algae Chlamydomonas reinhardtii cellular ultrastructure, with a particular emphasis on the different types of sample fixation that can be used, as well as compatible staining procedures including membrane. Basal body Cell architecture Chlamydomonas Chloroplast Expansion microscopy Flagella Membranes Microtubules Mitochondria Super-resolution microscopy Dewey 570 7daed2ee-1311-4824-9df9-2e51260a966a Département de biologie moléculaire et cellulaire 16484 false Bio-protocol 13 17 ccb43e13-32c5-4432-b7b1-62b3dc613b24 Article (Published version) Public BATMAN01.pdf 1494904 application/pdf CC BY-4.0 1fde9cbee6342d657ff588c42c8ad6cfef7b5e72485129b0109f781d7870b7e9 openAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire et cellulaire

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An In-depth Guide to the Ultrastructural Expansion Microscopy (U-ExM) of Chlamydomonas reinhardtii	Bio-protocol			2023	69	213

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Batman, Umut