Rapid characterization of aquatic hyphomycetes by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Article Article scientifique Article Rapid characterization of aquatic hyphomycetes by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry eng 2019-01-14 2019 author Cornut Julien a Laboratory of Applied Microbiology, University of Applied Sciences and Arts of Southern Switzerland , Via Mirasole 22A, 6501 Bellinzona , Switzerland.,b Institute of Earth Sciences, University of Applied Sciences and Arts of Southern Switzerland, Trevano Campus , 6952 Canobbio , Switzerland. 696098 author de Respinis Sophie 0000-0001-7260-7264 a Laboratory of Applied Microbiology, University of Applied Sciences and Arts of Southern Switzerland , Via Mirasole 22A, 6501 Bellinzona , Switzerland. author Tonolla Mauro Amedeo a Laboratory of Applied Microbiology, University of Applied Sciences and Arts of Southern Switzerland , Via Mirasole 22A, 6501 Bellinzona , Switzerland.,c Microbial Ecology Laboratory, Microbiology Unit, Department of Botany and Plant Biology , University of Geneva , Switzerland. 45173 Département des sciences végétales (BIVEG) author Petrini Orlando 0000-0002-1234-7832 d POLE Pharma Consulting , Breganzona , Switzerland. author Bärlocher Felix 0000-0003-2404-625X e Department of Biology , Mount Allison University , Sackville , News Brunswick E4L1G7, Canada. author Chauvet Eric 0000-0001-8676-392X f EcoLab, Université de Toulouse , CNRS, UPS, INPT, 31062 Toulouse , France. author Bruder Andreas 0000-0002-4591-491X a Laboratory of Applied Microbiology, University of Applied Sciences and Arts of Southern Switzerland , Via Mirasole 22A, 6501 Bellinzona , Switzerland.,b Institute of Earth Sciences, University of Applied Sciences and Arts of Southern Switzerland, Trevano Campus , 6952 Canobbio , Switzerland. 966299 177-189 10.1080/00275514.2018.1528129 30640580 0027-5514 Protein fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI--TOF MS) is a rapid, reliable, and economical method to characterize isolates of terrestrial fungi and other microorganisms. The objective of our study was to evaluate the suitability of MALDI-TOF MS for the identification of aquatic hyphomycetes, a polyphyletic group of fungi that play crucial roles in stream ecosystems. To this end, we used 34 isolates of 21 aquatic hyphomycete species whose identity was confirmed by spore morphology and internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) nuc rDNA sequencing. We tested the efficiency of three protein extraction methods, including chemical and mechanical treatments using 13 different protocols, with the objective of producing high-quality MALDI-TOF mass spectra. In addition to extraction protocols, mycelium age was identified as a key parameter affecting protein extraction efficiency. The dendrogram based on mass-spectrum similarity indicated good and relevant taxonomic discrimination; the tree structure was comparable to that of the phylogram based on ITS sequences. Consequently, MALDI-TOF MS could reliably identify the isolates studied and provided greater taxonomic accuracy than classical morphological methods. MALDI-TOF MS seems suited for rapid characterization and identification of aquatic hyphomycete species. Aquatic fungi ITS sequencing Mass spectrometry Morphology Phylogeny Proteomics Taxonomy Cluster Analysis France Fungal Proteins / analysis Mitosporic Fungi / chemistry Mitosporic Fungi / classification Water Microbiology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Dewey 580 45 Département des sciences végétales 163 false Mycologia 111 1 b80b5434-f249-4248-8650-279b32c3b1d7 Article (Published version) Restricted Rapid characterization of aquatic h1.pdf 1838357 application/pdf c3f16a7a45fdfc266bbd7d541fd430e6844475387cb4d3f932aa827de9cfb34f restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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Rapid characterization of aquatic hyphomycetes by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	Mycologia			2019	77	0

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Bruder, Andreas