The role of PQN-59 in cell division and protein homeostasis in C. elegans early development

Diplôme Thèse The role of PQN-59 in cell division and protein homeostasis in C. elegans early development eng 2025-04-09 author Pizzella Adriano 959276 director Gotta Monica 0000-0002-4336-7860 659510 Département de physiologie cellulaire et métabolisme (PHYME) 183 10.13097/archive-ouverte/unige:189715 urn:nbn:ch:unige-1897158 Sc. Vie 364 Cell division and protein homeostasis are fundamental processes required for organismal development. Disruption of either can lead to severe cellular and organismal abnormalities. In Caenorhabditis elegans, our laboratory identified PQN-59 as a component of stress granules and a key protein in early cell division and embryonic development. However, its precise function remains unclear. Here, we performed a structure–function analysis of PQN-59 to investigate a potential separation-of-function mutant between the aberrant first cell division and the stress granule localization phenotypes, with a focus on the loss of function allele pqn-59(ΔUBA). We also identify a novel PQN-59 interacting partner, SAO-1, whose human homologue GIGYF2 suggests a novel role for PQN-59 in protein homeostasis. Consistently, proteomic analyses revealed 70% of the differentially expressed proteins were downregulated in pqn-59(RNAi) and pqn-59(ΔUBA) conditions. Together, our findings establish PQN-59 as a critical regulator of early embryonic cell division and protein homeostasis in C. elegans. C. elegans Cell division Protein Homeostasis PQN-59 Dewey 612 134 Département de physiologie cellulaire et métabolisme 21C false 7e1d3ece-3f91-41e2-8e47-4c88a791ac05 Thesis Restricted AdrianoPizzella_PhDThesis.pdf 5003764 application/pdf 5b50750ba4352bafac0ecdac28985563315c09078473b44d42be121b1e802605 65253e59-462b-4fac-bbe5-d9de62c112c4 Imprimatur Public IMPRIMATUR_Adriano_PIZZELLA_signé_p.pdf 167592 application/pdf 40822b2cbca94f2371c1e27f0e84cf0b47b7e88b5a800c8064be1196e528e14f restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme

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The role of PQN-59 in cell division and protein homeostasis in C. elegans early development				2025	90	0

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Pizzella, Adriano

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