Patterning and growth control<i> in vivo</i> by an engineered GFP gradient

Article Article scientifique Article Patterning and growth control<i> in vivo</i> by an engineered GFP gradient eng 2020-10-16 author Stapornwongkul Kristina S. 0000-0002-7299-9672 The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK. author De Gennes Marc Pierre Gilles 0000-0003-1631-3231 The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK. 957126 author Cocconi Luca 0000-0002-8551-1461 The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK. 951609 author Salbreux Guillaume 0000-0001-7041-1292 The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK. 947792 Département de génétique et évolution (Genev) (BIANI) author Vincent Jean-Paul 0000-0003-2305-5744 The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK. 321-327 10.1126/science.abb8205 33060356 0036-8075 PMC7611032 https://www.science.org/doi/10.1126/science.abb8205 Morphogens provide positional information during tissue development. For this behavior to occur, morphogens must spread out and form a concentration gradient; however, their mechanism of transport remains a matter of debate. Stapornwongkul et al. now show that in the presence of extracellular binding elements (binders), the inert green fluorescent protein (GFP) can form a detectable concentration gradient by diffusion in the developing fly wing (see the Perspective by Barkai and Shilo). When combining the expression of nonsignaling binders and receptors engineered to respond to GFP, a synthetic GFP gradient can substitute for a natural morphogen to organize growth and patterning. In related work, Toda et al. also show that GFP can be converted into a morphogen by providing anchoring interactions that tether the molecule, forming a gradient that can be recognized by synthetic receptors that activate gene expression. These synthetic morphogens can be used to program de novo multidomain tissue patterns. These results highlight core mechanisms of morphogen signaling and patterning and provide ways to program spatial tissue organization independently from endogenous morphogen pathways. Wellcome Trust Theoretical Physics of Biology Laboratory FC001317 Wellcome Trust Epithelial Cell Interactions Laboratory FC001204 Cancer Research UK 21144 NIH HHS P40 OD018537 The Francis Crick Institute 10204 true Science 370 6514 3c6e20f3-60b2-4b27-82e7-fc3130058c70 Article (Published version) Restricted science.abb8205.pdf 2532721 application/pdf 5eace62b4e1b581c5e4f0c8a0c6627041b4a5b56bf505c106995004e489c9652 restrictedAccess

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Patterning and growth control in vivo by an engineered GFP gradient	Science			2020	82	0

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De Gennes, Marc Pierre Gilles