Photoresponsive Nanocarriers Based on Lithium Niobate Nanoparticles for Harmonic Imaging and On-Demand Release of Anticancer Chemotherapeutics

Article Article scientifique Article Photoresponsive Nanocarriers Based on Lithium Niobate Nanoparticles for Harmonic Imaging and On-Demand Release of Anticancer Chemotherapeutics eng 2022-06-03 2022-06-03 author Gheata Adrian author Gaulier Geoffrey 749322 author Campargue Gabriel 861805 author Vuilleumier Jérémy author Kaiser Simon 845081 author Gautschi Ivan author Riporto Florian author Beauquis Sandrine author Staedler Davide 827614 author Diviani Dario 955236 author Bonacina Luigi 0000-0003-0476-4473 632447 author Gerber-Lemaire Sandrine 0000-0002-6519-2782 10.1021/acsnanoscienceau.1c00044 2694-2496 https://pubs.acs.org/doi/10.1021/acsnanoscienceau.1c00044 Nanoparticle-based drug delivery systems have the potential for increasing the efficiency of chemotherapeutics by enhancing the drug accumulation at specific target sites, thereby reducing adverse side effects and mitigating patient acquired resistance. In particular, photo-responsive nanomaterials have attracted much interest due to their ability to release molecular cargos on demand upon light irradiation. In some settings, they can also provide complementary information by optical imaging on the (sub)cellular scale. We herein present a system based on lithium niobate harmonic nanoparticles (LNO HNPs) for the decoupled multi-harmonic cell imaging and near-infrared light-triggered delivery of an erlotinib derivative (ELA) for the treatment of epidermal growth factor receptor (EGFR)-overexpressing carcinomas. The ELA cargo was covalently conjugated to the surface of silica-coated LNO HNPs through a coumarinyl photo-cleavable linker, achieving a surface loading of the active molecule of 27 nmol/mg NPs. The resulting nanoconjugates (LNO-CM-ELA NPs) were successfully imaged upon pulsed laser excitation at 1250 nm in EGFR-overexpressing human prostate cancer cells DU145 by detecting the second harmonic emission at 625 nm, in the tissue transparency window. Tuning the laser at 790 nm resulted in the uncaging of the ELA cargo as a result of the second harmonic emission of the inorganic HNP core at 395 nm. This protocol induced a significant growth inhibition in DU145 cells, which was only observed upon specific irradiation at 790 nm, highlighting the promising capabilities of LNO-CM-ELA NPs for theranostic applications. Erlotinib derivative EGFR-positive cells Harmonic nanoparticles Light-triggered uncaging Second harmonic generation Surface functionalization Controlled drug release Dewey 500.2 Swiss National Science Foundation DARE - DeepAndREsolved 200021E_205745 CH Project funding 35 Groupe de physique appliquée 135 false 676 Groupe Wolf ACS nanoscience Au b5319ec6-e6a0-443d-957e-2003e599e838 Article (Published version) Public Article-scientifique-from-crossref.pdf 4059676 application/pdf CC BY openAccess Faculté des sciences / Section de physique / Groupe de physique appliquée

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Photoresponsive Nanocarriers Based on Lithium Niobate Nanoparticles for Harmonic Imaging and On-Demand Release of Anticancer Chemotherapeutics	ACS nanoscience Au			2022	248	170

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