Photoreceptor-induced LHL4 protects the photosystem II monomer in Chlamydomonas reinhardtii

Article Article scientifique Article Photoreceptor-induced LHL4 protects the photosystem II monomer in Chlamydomonas reinhardtii eng 2025-02-13 author Dannay Marie Université Grenoble Alpes, CNRS, CEA, INRAE, Interdisciplinary Research Institute of Grenoble, Cell and Plant Physiology Laboratory, Grenoble 38000, France.,Department of Plant Sciences, Section of Biology, Faculty of Sciences, University of Geneva, Geneva 1211, Switzerland. 952388 author Bertin Chloé Université Grenoble Alpes, CNRS, CEA, INRAE, Interdisciplinary Research Institute of Grenoble, Cell and Plant Physiology Laboratory, Grenoble 38000, France. author Cavallari Eva 0009-0001-5425-5338 Université Grenoble Alpes, CNRS, CEA, INRAE, Interdisciplinary Research Institute of Grenoble, Cell and Plant Physiology Laboratory, Grenoble 38000, France. author Albanese Pascal Université Grenoble Alpes, CNRS, CEA, INRAE, Interdisciplinary Research Institute of Grenoble, Cell and Plant Physiology Laboratory, Grenoble 38000, France.,Université Grenoble Alpes, CEA, INSERM, UA13 BGE, CNRS, CEA, Grenoble FR2048, France. author Tolleter Dimitri Université Grenoble Alpes, CNRS, CEA, INRAE, Interdisciplinary Research Institute of Grenoble, Cell and Plant Physiology Laboratory, Grenoble 38000, France. author Giustini Cécile Université Grenoble Alpes, CNRS, CEA, INRAE, Interdisciplinary Research Institute of Grenoble, Cell and Plant Physiology Laboratory, Grenoble 38000, France. author Menneteau Mathilde Université Grenoble Alpes, CNRS, CEA, INRAE, Interdisciplinary Research Institute of Grenoble, Cell and Plant Physiology Laboratory, Grenoble 38000, France. author Brugière Sabine Université Grenoble Alpes, CEA, INSERM, UA13 BGE, CNRS, CEA, Grenoble FR2048, France. author Couté Yohann 0000-0003-3896-6196 Université Grenoble Alpes, CEA, INSERM, UA13 BGE, CNRS, CEA, Grenoble FR2048, France. author Finazzi Giovanni 0000-0003-0597-7075 Université Grenoble Alpes, CNRS, CEA, INRAE, Interdisciplinary Research Institute of Grenoble, Cell and Plant Physiology Laboratory, Grenoble 38000, France. author Demarsy Emilie 0000-0002-0638-0812 Department of Plant Sciences, Section of Biology, Faculty of Sciences, University of Geneva, Geneva 1211, Switzerland. 867450 Département des sciences végétales (BIVEG) author Ulm Roman 0000-0001-8014-7392 Department of Plant Sciences, Section of Biology, Faculty of Sciences, University of Geneva, Geneva 1211, Switzerland.,Institute of Genetics and Genomics of Geneva, University of Geneva, Geneva 1211, Switzerland. 732804 Département des sciences végétales (BIVEG) author Allorent Guillaume 0000-0001-7414-139X Université Grenoble Alpes, CNRS, CEA, INRAE, Interdisciplinary Research Institute of Grenoble, Cell and Plant Physiology Laboratory, Grenoble 38000, France. e2418687122 10.1073/pnas.2418687122 39946539 0027-8424 PMC11848305 Photosynthesis, the fundamental process using light energy to convert carbon dioxide to organic matter, is vital for life on Earth. It relies on capturing light through light-harvesting complexes (LHC) in photosystem I (PSI) and PSII and on the conversion of light energy into chemical energy. Composition and organization of PSI and PSII core complexes are well conserved across evolution. PSII is particularly sensitive to photodamage but benefits from a large diversity of photoprotective mechanisms, finely tuned to handle the dynamic and ever-changing light conditions. Light Harvesting Complex protein family members (LHC and LHC-like families) have acquired a dual function during evolution. Members of the LHC antenna complexes of PS capture light energy, whereas others dissipate excess energy that cannot be harnessed for photosynthesis. This process mainly occurs through nonphotochemical quenching (NPQ). In this work, we focus on the Light Harvesting complex-Like 4 (LHL4) protein, a LHC-like protein induced by ultraviolet-B (UV-B) and blue light through UV Resistance locus 8 (UVR8) and phototropin photoreceptor-activated signaling pathways in the model green microalgae Chlamydomonas reinhardtii. We demonstrate that alongside established NPQ effectors, LHL4 plays a key role in photoprotection, preventing singlet oxygen accumulation in PSII and promoting cell survival upon light stress. LHL4 protective function is distinct from that of NPQ-related proteins, as LHL4 specifically and uniquely binds to the transient monomeric form of the core PSII complex, safeguarding its integrity. LHL4 characterization expands our understanding of the interplay between light harvesting and photoprotection mechanisms upon light stress in photosynthetic microalgae. Acclimation High light Photosynthesis Ultraviolet-B Chlamydomonas reinhardtii / genetics Chlamydomonas reinhardtii / metabolism Chlamydomonas reinhardtii / radiation effects Light Light-Harvesting Protein Complexes / genetics Light-Harvesting Protein Complexes / metabolism Photosystem II Protein Complex / genetics Photosystem II Protein Complex / metabolism Dewey 590 Dewey 580 IDEX Universite; Grenoble Alpes International Strategix Partnership SUnsPOT Centre National de la Recherche Scientifique (CNRS) Momentum 2018 Agence Nationale de la Recherche (ANR) ANR-15-IDEX-02 Grenoble Alliance for Structural Biology (GRAL) ANR-17-EURE-0003 Agence Nationale de la Recherche (ANR) ANR-10-INBS-08 Swiss National Science Foundation Perception and signalling of UV-B radiation in plants 207716 CH Projects European Commission Harnessing the Sun: Structural elucidation of the photoprotective Photosystem II interactome 101066400 PHOTO-LINK EU HE European Commission Chloroplast and Mitochondria interactions for microalgal acclimation 833184 ChloroMito EU H2020 45 Département des sciences végétales 163 23 Institut de génétique et de génomique 0DH false 832 Groupe Ulm https://www.ebi.ac.uk/pride/archive/projects/PXD048496 https://www.ebi.ac.uk/pride/archive/projects/PXD055508 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE255943 https://zenodo.org/records/14262714 Proceedings of the National Academy of Sciences of the United States of America 122 7 49428cd1-13ec-47cf-89f7-66a2a29a1958 Article (Published version) Public Article_scientifique_from_europePmc.pdf 3924153 application/pdf CC BY-NC-ND-4.0 7feaf0b692aaf4481b059700ae8b071f631e3dcb5f22602570d740999c798a45 fcbb3f6f-e38f-4060-9d96-164fc3c7250a Appendix Public pnas.2418687122.sapp.pdf 2860106 application/pdf 7a2941d0bd6303b83e84ac9f835559b5b6a655b89273b247a7f8cd5d557848bb 9c6ce18d-b9c2-4216-aeca-68d2a483ef68 Appendix Public pnas.2418687122.sd01.xlsx 338488 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 08f46ade969ceaadd90efcda02677b899cad839a7db58c74fd062088162170e7 ada5808b-1402-464f-b51d-2c3b8dae9425 Appendix Public pnas.2418687122.sd02.xlsx 701566 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 489e1b35277c0399bd92e5b2630736781d55b35dd943fe7c6ab55daa55b9a1e1 cea336f6-99ee-47eb-a91e-944cc2486310 Appendix Public pnas.2418687122.sd03.xlsx 13828 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet ac0c2365e9c8d1fc1fc58840c6a6176872faf5f8b389ca4f118b09d9ed73694f 4e1b26df-0dae-40ed-a699-0ef8715370fe Appendix Public pnas.2418687122.sd04.xlsx 342802 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 1f3a59632ca27bb9d90f53cf69832f271d95522de38f69f9ff99ddd7740a6ca2 openAccess Faculté des sciences / Section de biologie / Département des sciences végétales Centres et instituts / Institut de génétique et de génomique

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Photoreceptor-induced LHL4 protects the photosystem II monomer in Chlamydomonas reinhardtii	Proceedings of the National Academy of Sciences of the United States of America			2025	22	110

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Dannay, Marie