Extracellular and intracellular destruction of Pseudomonas aeruginosa by Dictyostelium discoideum phagocytes mobilize different antibacterial mechanisms

Article Article scientifique Article Extracellular and intracellular destruction of Pseudomonas aeruginosa by Dictyostelium discoideum phagocytes mobilize different antibacterial mechanisms eng 2023-11-28 2024-01 author Ayadi Imen 0000-0002-3901-7227 Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland. 832806 author Lamrabet Otmane Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland. 862612 author Munoz-Ruiz Raphaël Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland. 948000 Département de physiologie cellulaire et métabolisme (PHYME) author Jauslin Tania Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland. 850400 Structure enseignement (ENSI) author Guilhen Cyril Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland. 884610 Structure enseignement (ENSI) author Cosson Pierre 0000-0002-4881-0358 Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Geneva, Switzerland. 119596 Département de physiologie cellulaire et métabolisme (PHYME) 69-84 10.1111/mmi.15197 38017607 0950-382X https://onlinelibrary.wiley.com/doi/10.1111/mmi.15197 Ingestion and killing of bacteria by phagocytic cells are critical processes to protect the human body from bacterial infections. In addition, some immune cells (neutrophils, NK cells) can release microbicidal molecules in the extracellular medium to eliminate non-ingested microorganism. Molecular mechanisms involved in the resulting intracellular and extracellular killing are still poorly understood. In this study, we used the amoeba Dictyostelium discoideum as a model phagocyte to investigate the mechanisms allowing intracellular and extracellular killing of Pseudomonas aeruginosa. When a D. discoideum cell establishes a close contact with a P. aeruginosa bacterium, it can either ingest it and kill it in phagosomes, or kill it extracellularly, allowing a direct side-by-side comparison of these two killing modalities. Efficient intracellular destruction of P. aeruginosa requires the presence of the Kil2 pump in the phagosomal membrane. On the contrary, extracellular lysis is independent on Kil2 but requires the expression of the superoxide-producing protein NoxA, and the extracellular release of the AplA bacteriolytic protein. These results shed new light on the molecular mechanisms allowing elimination of P. aeruginosa bacteria by phagocytic cells. Dictyostelium discoideum Pseudomonas aeruginosa AplA Kil2 NADPH oxidase NoxA Amoebapore Bactericidal Killing Dewey 612 Swiss National Science Foundation Killing of bacteria in phagosomes: biochemistry, genetics and specificity. 201186 CH Projects 134 Département de physiologie cellulaire et métabolisme 21C false 111 Phagocytose 140 https://yareta.unige.ch/archives/3e0e3346-653f-4c20-9ed1-6dc349ba1b24 Molecular microbiology 121 1 9b8e2abe-b937-4922-bc78-3ae03b35e7e5 Article (Published version) Public Molecular_Microbiology_-_2023_-_Aya.pdf 2254710 application/pdf CC BY-4.0 1e0857885be7c5a3e15cd6c604ae9ba3878530dba741c4633ba2f42268ab092b 0d7bff42-94fe-47fe-b65f-18b188e72e54 Supplemental data Public mmi15197-sup-0001-figures1.pdf 19391 application/pdf 514dbaa100bdfc8701606179e6260b3eec8943f959df57783a3beb3941ac6337 6e14b7cc-037b-4b1e-bfbf-076667c7fbbc Supplemental data Public mmi15197-sup-0002-figures2.pdf 15756 application/pdf 6a9a7e5dc7c19e58a869b20583c9abcb8b46615bb02e605dab33044318ea7ac8 e77bd1cb-dd1d-465c-bba0-2d96725d1407 Supplemental data Public mmi15197-sup-0003-figures3.pdf 30565 application/pdf bb5e62955094f3f8c329c1623420ebcff3dbfa006509b47433729f8c685fce54 4413e378-6bae-4763-ba0b-b478e4c538de Supplemental data Public mmi15197-sup-0004-figures4.pdf 600901 application/pdf 37e821b91e2694202069637cc293ae1f76d7982ab48b96213cdfc346f12215c9 0a0bb30a-ba29-4c29-93ce-9f58f6d21ee9 Supplemental data Public mmi15197-sup-0005-figures5.pdf 2392914 application/pdf b96519318bd85669603d563b9638d4aa98b694f2db437ae00fb261c7ac5511d1 759337a7-9ad4-42ea-a774-c8bfbbf52b7e Supplemental data Public mmi15197-sup-0006-figures6.pdf 309495 application/pdf fec804f76698bf739e3015be355d4fb2c02b59d484d4f32ac216baee533e7f35 08f9266d-3f04-4dde-abf0-9a837de6069f Supplemental data Public mmi15197-sup-0007-figures7.pdf 29954 application/pdf e1621fee195c7e92a8f9fc3c490f4b1c776249167524dffbfe6cd04219041fde Vignette Public mmi15197-toc-0001-m.jpg 145661 image/png openAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme

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Extracellular and intracellular destruction of Pseudomonas aeruginosa by Dictyostelium discoideum phagocytes mobilize different antibacterial mechanisms	Molecular microbiology			2024	70	153

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Munoz-Ruiz, Raphaël