Role of two dileucine-like motifs in insulin receptor anchoring to microvilli

Article Article scientifique Article Role of two dileucine-like motifs in insulin receptor anchoring to microvilli eng 2002 author Shackleton Sue author Hamer Isabelle 28917 author Foti Michelangelo 0000-0001-7199-4135 28033 author Zumwald Nicole 94647 author Maeder Garavaglia Christine 22210 author Carpentier Jean-Louis 25558 43631-43637 10.1074/jbc.M204036200 12218050 0021-9258 In the absence of ligand, the insulin receptor is maintained on microvilli on the cell surface. A dileucine motif (LL(986-987)) is necessary but not sufficient for this anchoring, which also required the presence of additional sequence(s) downstream of position 1000. The aim of the present study was to identify this (these) additional sequence(s). First, exons 16 or 17 were fused to the extracellular and transmembrane domains of complement receptor 1 and stably expressed in Chinese hamster ovary cells. Results obtained indicate that exon 17 is sufficient for anchoring to microvilli. Second, analysis of insulin receptor mutants truncated within exon 17 demonstrated that whereas receptors truncated at position 1000 showed no preferential association with microvilli, receptors truncated at position 1012 displayed a level of association identical to that of the full-length insulin receptor. Third, mutation of a diisoleucine motif (II(1006-1007)) present within this 12-amino acid stretch abrogated the preferential association of the receptor with microvilli. These results indicate that the domain required for association of insulin receptor with microvilli is contained within the region encoded by exon 17 and that, within this sequence, two dileucine-like motifs (LL(986-987) and II(1006-1007)) play a crucial role. Amino Acid Motifs Animals CHO Cells Cells Cultured Cricetinae Cytoskeleton/metabolism DNA Complementary/metabolism Exons Leucine/chemistry Ligands Microscopy Electron Microvilli/ultrastructure Models Biological Mutation Protein Binding Protein Conformation Protein Structure Secondary Protein Structure Tertiary Receptor Insulin/chemistry/metabolism Temperature Time Factors Dewey 612 134 Département de physiologie cellulaire et métabolisme 21C false 472 Obésité et syndrome métabolique 803 The Journal of biological chemistry 277 46 e6c8b160-2c6f-49cd-8629-b3e5d4cfa758 Article (Published version) Restricted Shackleton et al JBC 2002.pdf 292085 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme

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Role of two dileucine-like motifs in insulin receptor anchoring to microvilli	The Journal of biological chemistry			2002	476	0

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Zumwald, Nicole