Adipose-derived stromal cells within a gelatin matrix acquire enhanced regenerative and angiogenic properties: a pre-clinical study for application to chronic wounds

Article Article scientifique Article Adipose-derived stromal cells within a gelatin matrix acquire enhanced regenerative and angiogenic properties: a pre-clinical study for application to chronic wounds eng 2023-03-22 2023-03-22 author Brembilla Nicolo 703016 Département de médecine (DEMED) author Modarressi Ghavami Seyed Ali 105647 Département de chirurgie (CHIRU) author André-Lévigne Dominik 0000-0002-0396-7511 748833 Département de chirurgie (CHIRU) author Brioudes Estelle 743625 author Lanza Florian 944544 author Vuagnat Hubert Blaise 32942 author Durual Stéphane 0000-0002-9772-8406 592156 CUMD - Biomatériaux (BMAT) author Marger Laurine 873227 CUMD - Biomatériaux (BMAT) author Boehncke Wolf-Henning 779122 Département de médecine (DEMED) author Krause Karl-Heinz 0000-0002-9033-6768 23022 Département de pathologie et immunologie (PATIM) author Preynat-Seauve Olivier 561776 Département de médecine (DEMED) 987 10.3390/biomedicines11030987 36979966 2227-9059 https://www.mdpi.com/2227-9059/11/3/987 <p>This study evaluates the influence of a gelatin sponge on adipose-derived stromal cells (ASC). Transcriptomic data revealed that, compared to ASC in a monolayer, a cross-linked porcine gelatin sponge strongly influences the transcriptome of ASC. Wound healing genes were massively regulated, notably with the inflammatory and angiogenic factors. Proteomics on conditioned media showed that gelatin also acted as a concentrator and reservoir of the regenerative ASC secretome. This secretome promoted fibroblast survival and epithelialization, and significantly increased the migration and tubular assembly of endothelial cells within fibronectin. ASC in gelatin on a chick chorioallantoic membrane were more connected to vessels than an empty sponge, confirming an increased angiogenesis in vivo. No tumor formation was observed in immunodeficient nude mice to which an ASC gelatin sponge was transplanted subcutaneously. Finally, ASC in a gelatin sponge prepared from outbred rats accelerated closure and re-vascularization of ischemic wounds in the footpads of rats. In conclusion, we provide here preclinical evidence that a cross-linked porcine gelatin sponge is an optimal carrier to concentrate and increase the regenerative activity of ASC, notably angiogenic. This formulation of ASC represents an optimal, convenient and clinically compliant option for the delivery of ASC on ischemic wounds.</p> Adipose stem cells Clinical translation Mesenchymal stem cells Stromal cells Dewey 617.6 Dewey 616.07 Dewey 616 Dewey 617 211 Biomatériaux 24Q22 137 Département de pathologie et immunologie 21F 56 Département de médecine 221 57 Département de chirurgie 222 false 239 Groupe Susanne Scherrer : Unité des biomatériaux 315 950 Génomique et métagénomique des produits sanguins labiles 956 38 Radicaux libres et cellules souches embryonnaires 60 576 Inflammation cutanée 919 Biomedicines 11 3 e009a77d-0fa8-4dd2-bf39-891faff7b849 Article (Published version) Public Article-scientifique-from-crossref.pdf 3936153 application/pdf CC BY-4.0 bd4848ace131c603b52434fdfe928ff43a2e36c6e0ec11c4a55a0a76f01ffd01 570e3f58-9b88-418a-8489-b0c0e1fe2def Supplemental data Public biomedicines-2273784-supplementary.pdf 391438 application/pdf CC BY-4.0 86c549e0ac05b6187b9564add3f2f71c9cb5f47f00e9046cada3c9bb2ec1d602 openAccess Faculté de médecine / Section de médecine dentaire / Département de réhabilitation oro-faciale / Division de prothèse fixe et biomatériaux / Biomatériaux Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine clinique / Département de chirurgie

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Adipose-derived stromal cells within a gelatin matrix acquire enhanced regenerative and angiogenic properties: a pre-clinical study for application to chronic wounds	Biomedicines			2023	169	108

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