The Sendai paramyxovirus accessory C proteins inhibit viral genome amplification in a promoter-specific fashion

Article Article scientifique Article The Sendai paramyxovirus accessory C proteins inhibit viral genome amplification in a promoter-specific fashion eng 1996 author Cadd Tamarra Leigh 93842 author Garcin Dominique 24296 author Tapparel Caroline 0000-0002-0411-6567 27039 author Itoh M author Homma M author Roux Laurent 24751 author Curran Joseph 22465 author Kolakofsky Daniel 25420 5067-5074 8764014 0022-538X Many paramyxoviruses express small basic C proteins, from an alternate, overlapping open reading frame of the P gene mRNA, which were previously found to inhibit mRNA synthesis. During recent experiments in which infectious Sendai virus (SeV) was recovered from cDNA via the initial expression of the viral N, P, and L genes from plasmids, the abrogation of C protein expression from the plasmid P gene was found to be necessary for virus recovery. We have investigated the effect of C coexpression on the amplification of an internally deleted defective interfering (DI) genome directly in the transfected cell, for which, in contrast to virus recovery experiments, genome amplification is independent of mRNA synthesis carried out by the SeV polymerase. We find that C protein coexpression also strongly inhibits the amplification of this DI genome but has little or no effect on that of a copy-back DI genome (DI-H4). We have also characterized the C protein from a mutant SeV and found that (i) it had lost most of its inhibitory activity on internally deleted DI genome amplification and (ii) its coexpression no longer prevented the recovery of SeV from DNA. However, consistent with the insensitivity of copy-back DI genomes to C protein inhibition, C coexpression did not prevent the recovery of copy-back nondefective viruses from DNA. The inhibitory effects of C coexpression thus appear to be promoter specific. Gene Amplification Gene Expression Regulation Genome, Viral Mutation Promoter Regions, Genetic/genetics Respirovirus/genetics/metabolism Viral Proteins/genetics Dewey 616 54 Département de microbiologie et médecine moléculaire 21B false 365 Régulation traductionnelle dans les cellules de mammifère 631 216 Virologie moléculaire 275 Journal of virology 70 8 74050e63-3e1f-45e2-82db-71b49467cde7 Article (Published version) Public 705067.pdf 406437 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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The Sendai paramyxovirus accessory C proteins inhibit viral genome amplification in a promoter-specific fashion	Journal of virology			1996	580	244

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Cadd, Tamarra Leigh