Harmonic Imaging of Stem Cells in Whole Blood at GHz Pixel Rate

Article Article scientifique Article Harmonic Imaging of Stem Cells in Whole Blood at GHz Pixel Rate eng 2024-06-11 2024-06-11 author Karpf Sebastian 0000-0002-7665-5914 Institute of Biomedical Optics (BMO) University Of Luebeck 23562 Luebeck Germany author Glöckner Burmeister Nina Institute of Biomedical Optics (BMO) University Of Luebeck 23562 Luebeck Germany author Dubreil Laurence Oniris, INRAE, PAnther Nantes F‐44307 France author Ghosh Shayantani 0000-0003-0479-6248 Department of Applied Physics Université de Genève Rue de l'Ecole‐de‐Médecine, 20 Geneva 1205 Switzerland 937060 Serv com physique appliquée (FIGCO) author Hollandi Reka Synthetic and Systems Biology Unit Biological Research Centre (BRC) Szeged H‐6726 Hungary author Pichon Julien Oniris, INRAE, PAnther Nantes F‐44307 France author Leroux Isabelle Oniris, INRAE, PAnther Nantes F‐44307 France author Henkel Alessandra Institute of Biomedical Optics (BMO) University Of Luebeck 23562 Luebeck Germany author Lutz Valerie Institute of Biomedical Optics (BMO) University Of Luebeck 23562 Luebeck Germany author Jurkevičius Jonas Institute of Biomedical Optics (BMO) University Of Luebeck 23562 Luebeck Germany author Latshaw Alexandra Lane Department of Applied Physics Université de Genève Rue de l'Ecole‐de‐Médecine, 20 Geneva 1205 Switzerland 404426 Serv com physique appliquée (FIGCO) author Kilin Vasyl Department of Applied Physics Université de Genève Rue de l'Ecole‐de‐Médecine, 20 Geneva 1205 Switzerland 848858 author Kutscher Tonio Institute of Biomedical Optics (BMO) University Of Luebeck 23562 Luebeck Germany author Wiggert Moritz Department of Applied Physics Université de Genève Rue de l'Ecole‐de‐Médecine, 20 Geneva 1205 Switzerland author Saavedra‐Villanueva Oscar Oniris, INRAE, PAnther Nantes F‐44307 France author Vogel Alfred Institute of Biomedical Optics (BMO) University Of Luebeck 23562 Luebeck Germany author Huber Robert A. Institute of Biomedical Optics (BMO) University Of Luebeck 23562 Luebeck Germany author Horvath Peter Synthetic and Systems Biology Unit Biological Research Centre (BRC) Szeged H‐6726 Hungary author Rouger Karl Oniris, INRAE, PAnther Nantes F‐44307 France author Bonacina Luigi 0000-0003-0476-4473 Department of Applied Physics Université de Genève Rue de l'Ecole‐de‐Médecine, 20 Geneva 1205 Switzerland 632447 Serv com physique appliquée (FIGCO) 2401472 10.1002/smll.202401472 1613-6810 https://onlinelibrary.wiley.com/doi/10.1002/smll.202401472 <p>The pre‐clinical validation of cell therapies requires monitoring the biodistribution of transplanted cells in tissues of host organisms. Real‐time detection of these cells in the circulatory system and identification of their aggregation state is a crucial piece of information, but necessitates deep penetration and fast imaging with high selectivity, subcellular resolution, and high throughput. In this study, multiphoton‐based in‐flow detection of human stem cells in whole, unfiltered blood is demonstrated in a microfluidic channel. The approach relies on a multiphoton microscope with diffractive scanning in the direction perpendicular to the flow via a rapidly wavelength‐swept laser. Stem cells are labeled with metal oxide harmonic nanoparticles. Thanks to their strong and quasi‐instantaneous second harmonic generation (SHG), an imaging rate in excess of 10 000 frames per second is achieved with pixel dwell times of 1 ns, a duration shorter than typical fluorescence lifetimes yet compatible with SHG. Through automated cell identification and segmentation, morphological features of each individual detected event are extracted and cell aggregates are distinguished from isolated cells. This combination of high‐speed multiphoton microscopy and high‐sensitivity SHG nanoparticle labeling in turbid media promises the detection of rare cells in the bloodstream for assessing novel cell‐based therapies.</p> Dewey 500.2 35 Groupe de physique appliquée 135 false 1414 Groupe Bonacina Small 7f46f301-3c25-4c00-9ba4-3dc89310ad0d Article (Accepted version) Public Karpf2024.pdf 4624553 application/pdf CC BY-NC-4.0 75604cde0e8873eba5a5536851ba8bb77517a5674cdc0337744cee3138af37b2 openAccess Faculté des sciences / Section de physique / Groupe de physique appliquée

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