Neurofibromin 1 in mushroom body neurons mediates circadian wake drive through activating cAMP

Article Article scientifique Article Neurofibromin 1 in mushroom body neurons mediates circadian wake drive through activating cAMP–PKA signaling eng 2021-10-01 2021-10-01 author Machado Almeida Pedro 0000-0003-4423-7707 675906 author Lago Solis Blanca 924340 Département de génétique et évolution (Genev) (BIANI) author Stickley Luca 0000-0001-6344-3122 813828 author Feidler Alexis 0000-0002-1827-6473 917339 author Nagoshi Emi 0000-0002-6365-4074 535940 Département de génétique et évolution (Genev) (BIANI) 1-17 5758 10.1038/s41467-021-26031-2 34599173 2041-1723 PMC8486785 https://www.nature.com/articles/s41467-021-26031-2 Various behavioral and cognitive states exhibit circadian variations in animals across phyla including Drosophila melanogaster , in which only ~0.1% of the brain’s neurons contain circadian clocks. Clock neurons transmit the timing information to a plethora of non-clock neurons via poorly understood mechanisms. Here, we address the molecular underpinning of this phenomenon by profiling circadian gene expression in non-clock neurons that constitute the mushroom body, the center of associative learning and sleep regulation. We show that circadian clocks drive rhythmic expression of hundreds of genes in mushroom body neurons, including the Neurofibromin 1 ( Nf1 ) tumor suppressor gene and Pka-C1 . Circadian clocks also drive calcium rhythms in mushroom body neurons via NF1-cAMP/PKA-C1 signaling, eliciting higher mushroom body activity during the day than at night, thereby promoting daytime wakefulness. These findings reveal the pervasive, non-cell-autonomous circadian regulation of gene expression in the brain and its role in sleep. Dewey 590 Georges and Antoine Claraz Foundation, the EuroScholars program, the Plan Strategique Sciences Vie (PSVIE) of the University of Geneva MEXT | Japan Science and Technology Agency PRESTO(10101675) Swiss National Science Foundation Non-cell-autonomous circadian regulation of brain function 189169 CH Projects 44 Département de génétique et évolution 162 false Nature communications 12 8e42131d-31ad-42d3-ac24-19964eb5116a Article (Published version) Public Article-scientifique-from-unpaywall.pdf 3435309 application/pdf CC BY-4.0 8d9c0db076bef9d0808a68f5731fb8437d1371c81daa94a4ec65c17c8391acdb openAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

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Neurofibromin 1 in mushroom body neurons mediates circadian wake drive through activating cAMP–PKA signaling	Nature communications			2021	17	9

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