Studying the contribution of the Pseudomonas aeruginosa RNA degradosome in bacterial adaptation to cystic fibrosis lungs

Diplôme Master Studying the contribution of the Pseudomonas aeruginosa RNA degradosome in bacterial adaptation to cystic fibrosis lungs eng 2024-08-20 author Guex-Crosier Sylvain 923802 director Valentini Martina 0000-0002-1948-8339 889251 Département de microbiologie et médecine moléculaire (MIMOL) 60 Pseudomonas aeruginosa is known to cause chronic infections in Cystic Fibrosis (CF) patients by establishing itself in their lungs for extended periods. The RNA degradosome is an important regulatory machinery in bacteria that controls environmental adaptation by acting on RNA. The RNA degradosome main component, the endoribonuclease RNase E, acts as a scaffold for other RNA degradosome components while possessing its own catalytic activity. The composition and importance of the P. aeruginosa RNA degradosome during lung colonization in CF patients are not yet established. Here, we address these questions by using different RNase E mutants and an artificial sputum medium (ASM) that mimics the lung mucus of CF patients. First, we performed phenotypic analyses of the mutants in ASM and in the traditional laboratory medium NYB to compare the two media. Second, we searched for new components of the RNA degradosome in ASM, as the machinery composition changes according to the bacterial environment. Third, we set up a transposon-sequencing protocol that will allow to study the contribution of RNA degradosome assembly to bacterial fitness. Overall, we showed that P. aeruginosa RNA degradosome action and composition can be different according to the growing medium, which may give importance to studying bacteria in a medium that mimics the infectious environment. Dewey 616 Dewey 570 Master in Molecular Biosciences, Genetics, Development, and Evolution 30 Section de biologie 16 54 Département de microbiologie et médecine moléculaire 21B false 1384 RNA Métabolisme et pathogenèse bactérienne 1036 4d88c0d4-2c60-4607-a2a0-3121e09a2f6f Master thesis Private Master_Thesis_Sylvain_Guex-Crosier_.pdf 1383707 application/pdf 2006bf727c28bcd2f4a94511798d9d301cc1e466d8442f0064e28955b4d74531 closedAccess Faculté des sciences / Section de biologie Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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Studying the contribution of the Pseudomonas aeruginosa RNA degradosome in bacterial adaptation to cystic fibrosis lungs				2024	38	3

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Guex-Crosier, Sylvain

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