Ex Vivo Functional Benchmarking of Hyaluronan-Based Osteoarthritis Viscosupplement Products: Comprehensive Assessment of Rheological, Lubricative, Adhesive, and Stability Attributes

Article Article scientifique Article Ex Vivo Functional Benchmarking of Hyaluronan-Based Osteoarthritis Viscosupplement Products: Comprehensive Assessment of Rheological, Lubricative, Adhesive, and Stability Attributes eng 2023-10-09 2023-10-09 author Porcello Alexandre 0000-0003-1603-2704 School of Pharmaceutical Sciences, University of Geneva, CH-1206 Geneva, Switzerland.,Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CH-1206 Geneva, Switzerland. 802436 author Hadjab Farid Development Department, Albomed GmbH, D-90592 Schwarzenbruck, Germany. author Ajouaou Maryam School of Pharmaceutical Sciences, University of Geneva, CH-1206 Geneva, Switzerland.,Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CH-1206 Geneva, Switzerland. 922027 author Philippe Virginie Regenerative Therapy Unit, Lausanne University Hospital, University of Lausanne, CH-1066 Epalinges, Switzerland.,Orthopedics and Traumatology Service, Lausanne University Hospital, University of Lausanne, CH-1011 Lausanne, Switzerland. author Martin Robin 0000-0002-8314-7678 Orthopedics and Traumatology Service, Lausanne University Hospital, University of Lausanne, CH-1011 Lausanne, Switzerland. author Abdel-Sayed Philippe 0000-0002-6054-0573 Regenerative Therapy Unit, Lausanne University Hospital, University of Lausanne, CH-1066 Epalinges, Switzerland.,STI School of Engineering, Federal Polytechnic School of Lausanne, CH-1015 Lausanne, Switzerland. author Hirt-Burri Nathalie 0000-0003-1220-0213 Regenerative Therapy Unit, Lausanne University Hospital, University of Lausanne, CH-1066 Epalinges, Switzerland. author Scaletta Corinne Regenerative Therapy Unit, Lausanne University Hospital, University of Lausanne, CH-1066 Epalinges, Switzerland. author Raffoul Wassim 0000-0002-3524-0077 Regenerative Therapy Unit, Lausanne University Hospital, University of Lausanne, CH-1066 Epalinges, Switzerland. author Applegate Lee Ann 0000-0002-7346-9921 Regenerative Therapy Unit, Lausanne University Hospital, University of Lausanne, CH-1066 Epalinges, Switzerland.,Center for Applied Biotechnology and Molecular Medicine, University of Zurich, CH-8057 Zurich, Switzerland.,Oxford OSCAR Suzhou Center, Oxford University, Suzhou 215123, China. author Allémann Eric 0000-0002-6598-9521 School of Pharmaceutical Sciences, University of Geneva, CH-1206 Geneva, Switzerland.,Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CH-1206 Geneva, Switzerland. 26730 Section des sciences pharmaceutiques (FARMA) author Jordan Olivier 0000-0003-3752-2198 School of Pharmaceutical Sciences, University of Geneva, CH-1206 Geneva, Switzerland.,Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CH-1206 Geneva, Switzerland. 133859 Section des sciences pharmaceutiques (FARMA) author Laurent Alexis 0000-0001-5738-8702 Regenerative Therapy Unit, Lausanne University Hospital, University of Lausanne, CH-1066 Epalinges, Switzerland.,Manufacturing Department, LAM Biotechnologies SA, CH-1066 Epalinges, Switzerland. 808 10.3390/gels9100808 37888381 2310-2861 PMC10606320 While many injectable viscosupplementation products are available for osteoarthritis (OA) management, multiple hydrogel functional attributes may be further optimized for efficacy enhancement. The objective of this study was to functionally benchmark four commercially available hyaluronan-based viscosupplements (Ostenil, Ostenil Plus, Synvisc, and Innoryos), focusing on critical (rheological, lubricative, adhesive, and stability) attributes. Therefore, in vitro and ex vivo quantitative characterization panels (oscillatory rheology, rotational tribology, and texture analysis with bovine cartilage) were used for hydrogel product functional benchmarking, using equine synovial fluid as a biological control. Specifically, the retained experimental methodology enabled the authors to robustly assess and discuss various functional enhancement options for hyaluronan-based hydrogels (chemical cross-linking and addition of antioxidant stabilizing agents). The results showed that the Innoryos product, a niacinamide-augmented linear hyaluronan-based hydrogel, presented the best overall functional behavior in the retained experimental settings (high adhesivity and lubricity and substantial resistance to oxidative degradation). The Ostenil product was conversely shown to present less desirable functional properties for viscosupplementation compared to the other investigated products. Generally, this study confirmed the high importance of formulation development and control methodology optimization, aiming for the enhancement of novel OA-targeting product critical functional attributes and the probability of their clinical success. Overall, this work confirmed the tangible need for a comprehensive approach to hyaluronan-based viscosupplementation product functional benchmarking (product development and product selection by orthopedists) to maximize the chances of effective clinical OA management. Adhesivity Antioxidants Functional attributes Hyaluronic acid Hydrogel stability Lubrication Osteoarthritis Rheology Tribology Viscosupplementation Dewey 615 31 Section des sciences pharmaceutiques 17 false 879 Biopharmacie 883 Technologie pharmaceutique Gels 9 10 0de2d336-ea8f-43e4-b42f-361287ef78c9 Article (Published version) Public Article_scientifique_from_europePmc.pdf 3254351 application/pdf CC BY a5f5a5fbcf7adebf6a2205221df5b69288e3ef902923db3cfdc6373228a36122 cef5f6c5-ca87-4031-b0ad-46bbb8e9d3ac Supplemental data Public gels-2631337-supplementary.pdf 1048867 application/pdf 75ffec0bb70af01d34c23e12c4ae948e50b32e7f2eec21687f619163021089b6 openAccess Faculté des sciences / Section des sciences pharmaceutiques

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Ex Vivo Functional Benchmarking of Hyaluronan-Based Osteoarthritis Viscosupplement Products: Comprehensive Assessment of Rheological, Lubricative, Adhesive, and Stability Attributes	Gels			2023	62	43

Archive ouverte UNIGE

Ajouaou, Maryam