Non-linear imaging using a custom-built system with new laser sources in the short-wave infrared region (SWIM, from project FAIRCHARM)

Diplôme Master Non-linear imaging using a custom-built system with new laser sources in the short-wave infrared region (SWIM, from project FAIRCHARM) eng 2024-09-09 author Krähenbühl Thomas Marc 919462 director Bonacina Luigi 0000-0003-0476-4473 632447 Serv com physique appliquée (FIGCO) 69 <p>Over the past three decades, non-linear imaging techniques have emerged as remarkable imaging methods, pushing the boundaries of optical microscopy. Here, we showcase the capabilities of the short-wave infrared microscope (SWIM), which takes use of novel laser sources in the short-wave infrared region and was developed for the EU-funded FAIRCHARM (fast infrared coherent harmonic microscopy) project. To do so, we employed simultaneous detection in both forward and backward directions of fluorescent and harmonic signals at high spatial resolution, using 1300 and 1650 nm excitation wavelengths from various samples, including zebrafish larvae, zebrafish scales, porcine cardiac tissues and daphnia. Particular focus was given to imaging zebrafish scales, as we collaborate with researchers studying vertebrate bone regeneration. Together, we developed a method to precisely assess collagen fibres and their relationships with osteoblasts and the mineralized bone layer. Additionally, we began investigating Erk activity, mechanical cues in cells and their connections to collagen fibres.</p> Dewey 500.2 Dewey 570 30 Section de biologie 16 35 Groupe de physique appliquée 135 false 1414 Groupe Bonacina 1439 Groupe de Simone dd252d37-99af-4f4b-a0a8-eed3bba20f56 Master thesis Restricted Master's_thesis_TK.pdf 12155895 application/pdf 9f25e58f509d7f21672a81d670416906dc2edb09d31f63f32e06a5241de2c84e restrictedAccess Faculté des sciences / Section de biologie Faculté des sciences / Section de physique / Groupe de physique appliquée

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Non-linear imaging using a custom-built system with new laser sources in the short-wave infrared region (SWIM, from project FAIRCHARM)				2024	140	14

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Krähenbühl, Thomas

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