Respiratory tissue-associated commensal bacteria offer therapeutic potential against pneumococcal colonization

Article Article scientifique Article Respiratory tissue-associated commensal bacteria offer therapeutic potential against pneumococcal colonization eng 2020 author Yildiz Soner 830372 author Pereira Bonifacio Lopes Joao Pedro 0000-0001-5505-8435 918900 author Berge Matthieu 811798 author Gonzalez Ruiz Victor 831974 author Baud Damien 825371 author Kloehn Joachim 869011 author Carvalho Soares De Mesqui Inês 910478 author Schaeren Olivier author Schotsaert Michael author Hathaway Lucy J author Rudaz Serge 0000-0002-4180-5417 28978 author Viollier Patrick 0000-0002-5249-9910 706923 author Hapfelmeier Siegfried author Francois Patrice 0000-0002-0540-750X 28911 author Schmolke Mirco 0000-0002-2491-3029 821513 e53581 10.7554/eLife.53581 33287959 2050-084X Under eubiotic conditions commensal microbes are known to provide a competitive barrier against invading bacterial pathogens in the intestinal tract, on the skin or on the vaginal mucosa. Here, we evaluate the role of lung microbiota in Pneumococcus colonization of the lungs. In eubiosis, the lungs of mice were dominantly colonized by Lactobacillus murinus. Differential analysis of 16S rRNA gene sequencing or L. murinus-specific qPCR of DNA from total organ homogenates vs.broncho alveolar lavages implicated tight association of these bacteria with the host tissue. Pure L. murinus conditioned culture medium inhibited growth and reduced the extension of pneumococcal chains. Growth inhibition in vitro was likely dependent on L. murinus-produced lactic acid, since pH neutralization of the conditioned medium aborted the antibacterial effect. Finally, we demonstrate that L. murinus provides a barrier against pneumococcal colonization in a respiratory dysbiosis model after an influenza A virus infection, when added therapeutically. Dewey 615 Dewey 616 European Commission Molecular Basis of Toxoplasma gondii Encystation and Persistence 695596 ToxoPersist EU H2020 Swiss National Science Foundation Acid-base balance in chronic kidney disease 155959 CH Projects Swiss National Science Foundation Long-term consequences of microbiota alterations for host physiology after acute respiratory infections in childhood 182475 CH Projects Swiss National Science Foundation Cell cycle-regulated surface structures and mobile DNA in bacteria 182576 CH Projects 31 Section des sciences pharmaceutiques 17 54 Département de microbiologie et médecine moléculaire 21B false 338 Génomique et microbiote 604 912 Modulation de la flore microbienne dans un hôte infecté par un virus 947 554 Régulation du cycle cellulaire dans C.crescentus, une bactérie asymétrique 895 881 Sciences analytiques eLife 9 2793de4a-80c6-435b-a9a6-1e0c68361fa5 Article (Published version) Public elife-53581-v1.pdf 3469983 application/pdf CC BY ab4e12a5-27f9-443b-8994-7923bf90bcc4 Supplemental data Public elife-53581-supp1-v1.xlsx 1462294 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet CC BY e2cf18c5-48e7-4432-b9c3-5537d4785006 Supplemental data Public elife-53581-supp2-v1.coli.xlsx 21378 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet CC BY fbff2d96-b95e-4f92-9cc2-f9a30b93b9a7 Supplemental data Public elife-53581-supp3-v1.xlsx 7393025 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet CC BY openAccess Faculté des sciences / Section des sciences pharmaceutiques Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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Respiratory tissue-associated commensal bacteria offer therapeutic potential against pneumococcal colonization	eLife			2020	455	218

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