Suberin plasticity to developmental and exogenous cues is regulated by a set of MYB transcription factors

Article Article scientifique Article Suberin plasticity to developmental and exogenous cues is regulated by a set of MYB transcription factors eng 2021-09-28 author Shukla Vinay 0000-0002-7838-8565 Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland. 908857 author Han Jianpu 0000-0002-7289-1856 Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland. 920244 Département des sciences végétales (BIVEG) author Cleard Karch Fabienne Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland. 23778 Département des sciences végétales (BIVEG) author Legendre Lefebvre Linnka 0000-0001-7827-0834 Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland. 134661 Département des sciences végétales (BIVEG) author Gully Kay 0000-0001-7968-3059 Department of Molecular Plant Biology, University of Lausanne, 1015 Lausanne, Switzerland. author Flis Paulina 0000-0002-5529-7599 Future Food Beacon of Excellence and School of Biosciences, University of Nottingham, NG7 2RD Nottingham, United Kingdom. author Berhin Alice 0000-0002-5214-6533 Department of Molecular Plant Biology, University of Lausanne, 1015 Lausanne, Switzerland. author Andersen Tonni G 0000-0002-8905-0850 Department of Molecular Plant Biology, University of Lausanne, 1015 Lausanne, Switzerland. author Salt David E Future Food Beacon of Excellence and School of Biosciences, University of Nottingham, NG7 2RD Nottingham, United Kingdom. author Nawrath Christiane 0000-0003-1470-8459 Department of Molecular Plant Biology, University of Lausanne, 1015 Lausanne, Switzerland. author Barberon Marie 0000-0002-8169-8580 Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland; marie.barberon@unige.ch. 898432 Département des sciences végétales (BIVEG) e2101730118 10.1073/pnas.2101730118 34551972 0027-8424 PMC8488582 Suberin is a hydrophobic biopolymer that can be deposited at the periphery of cells, forming protective barriers against biotic and abiotic stress. In roots, suberin forms lamellae at the periphery of endodermal cells where it plays crucial roles in the control of water and mineral transport. Suberin formation is highly regulated by developmental and environmental cues. However, the mechanisms controlling its spatiotemporal regulation are poorly understood. Here, we show that endodermal suberin is regulated independently by developmental and exogenous signals to fine-tune suberin deposition in roots. We found a set of four MYB transcription factors (MYB41, MYB53, MYB92, and MYB93), each of which is individually regulated by these two signals and is sufficient to promote endodermal suberin. Mutation of these four transcription factors simultaneously through genome editing leads to a dramatic reduction in suberin formation in response to both developmental and environmental signals. Most suberin mutants analyzed at physiological levels are also affected in another endodermal barrier made of lignin (Casparian strips) through a compensatory mechanism. Through the functional analysis of these four MYBs, we generated plants allowing unbiased investigation of endodermal suberin function, without accounting for confounding effects due to Casparian strip defects, and were able to unravel specific roles of suberin in nutrient homeostasis. ABA CIF Endodermis Root Suberin Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / metabolism Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Lipids / physiology Proto-Oncogene Proteins c-myb / genetics Proto-Oncogene Proteins c-myb / metabolism Transcription Factors / genetics Transcription Factors / metabolism Dewey 580 Swiss National Science Foundation Connecting molecular structure with function of cutin and other cell-wall associated polyesters 188672 CH Projects 45 Département des sciences végétales 163 false Proceedings of the National Academy of Sciences of the United States of America 118 39 67171ad3-8ca4-4f3d-a5ac-e61cb4999960 Appendix Public pnas.2101730118.sapp.pdf 2832013 application/pdf bf4f07f468730e384f78cb22220fc4b9c8ae646ff10c4ce2058bfcb06787c322 a6e7c85d-ccf0-4dbf-8917-f406e26173b8 Article (Published version) Public Article-scientifique-from-europePmc.pdf 1770282 application/pdf CC BY-NC-ND 8ba42458616f311e272b1dece910597802c3cb17a99fb2182314ef982dc3709c openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

Title	Published in	Year	Views	Downloads

Suberin plasticity to developmental and exogenous cues is regulated by a set of MYB transcription factors	Proceedings of the National Academy of Sciences of the United States of America	2021	114	219
Building and breaking of a barrier: Suberin plasticity and function in the endodermis	Current opinion in plant biology	2021	82	949

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Shukla, Vinay