Tissue Engineering In Vitro Leaflet- and 3-Dimensional Printing-Based Implant Prototypes for Infant Mitral Valve

Article Article scientifique Article Tissue Engineering In Vitro Leaflet- and 3-Dimensional Printing-Based Implant Prototypes for Infant Mitral Valve eng 2025-08-07 2025 author González-Duque Martha I. author Breuninger Arielle author Leis Frédéric Jonathan 907387 author Michaud Julio B. author Sivakumar Shaginth author Pautu Vincent author Jaconi Dévaud Marisa 0000-0002-2004-8226 23312 Département de neurosciences fondamentales (NEUFO) Marisa Jaconi author Jobin Marc author Roux Adrien 0159 10.34133/bmef.0159 40777733 2765-8031 PMC12329791 https://spj.science.org/doi/10.34133/bmef.0159 Objective: This study engineers leaflet- and 3-dimensional (3D) printing-based implant prototypes for infant mitral valve repair via in vitro cultured mesoangioblasts isolated from the human fetal aorta (AoMAB). Impact Statement: Ultrahigh-molecular-weight polyethylene (UHMWPE) coatings, as well as 3D-printed gelatin methacrylate (GelMA) hydrogels for implants, represent new possibilities for devices used in mitral valve repair. Introduction: Mitral valve prolapse (MVP) repair in pediatric patients is challenging due to somatic growth, patient–prosthesis mismatch, reinterventions, infections, and thromboembolism. Tissue-engineered heart valves (TEHVs) offer potential solutions through conventional and 3D printing biofabrication. Methods: Four materials are evaluated: UHMWPE, UHMWPE coated with polyvinyl alcohol (PVA), UHMWPE coated with PVA and collagen, and 3D-printed GelMA hydrogels. The prototypes are characterized for micro/nanostructural, physicochemical (degradation, contact angle, Fourier transform infrared spectroscopy), and mechanical properties (simple strength tests, dynamic mechanical analysis) and assessed for cytocompatibility using AoMAB cells. A 3D printing mitral valve prototype is analyzed via immunostaining. Results: Results highlight UHMWPE coated with PVA and collagen as the most promising, with degradation (7.30 ± 18.71%), a hydrophilic contact angle (26.13 ± 1.45°), and biocompatibility (177.04 ± 68.92% viability). GelMA prototypes show superior viability (216.77 ± 77.69%) and scalability for 3D printing. Conclusion: UHMWPE coated with PVA and collagen and GelMA demonstrate strong potential for TEHVs, with AoMAB cells facilitating 3D culture and future personalized pediatric applications. Further in vitro validation and thrombogenicity assessments are needed. Dewey 616.8 53 Département de neurosciences fondamentales 21A false 281 Mécanismes de différentiation cellulaire des cellules cardiaques 536 BME frontiers 6 dac6533c-78d0-4e53-ba24-906103102ebe Article (Published version) Public González-Duque_BMEF_2025.pdf 799778 application/pdf CC BY-4.0 a72f0282001e707c22ffec8bc579d674254295b8dffed248247963470032d965 openAccess Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Tissue Engineering In Vitro Leaflet- and 3-Dimensional Printing-Based Implant Prototypes for Infant Mitral Valve	BME frontiers			2025	21	178

Archive ouverte UNIGE

Leis, Frédéric Jonathan

account_balance UNIGE affiliation entities

UNIGE affiliation entities