Isolation and characterization of a cDNA clone encoding an osmotin-like protein from <i>Arabidopsis thaliana</i>

Article Article scientifique Article Isolation and characterization of a cDNA clone encoding an osmotin-like protein from <i>Arabidopsis thaliana</i> eng 1997 author Capelli Nicolas 26677 author Diogon Thierry Robert René 90557 author Greppin Hubert 24907 author Simon Patrice 26360 51-56 10.1016/S0378-1119(97)00029-2 0378-1119 https://linkinghub.elsevier.com/retrieve/pii/S0378111997000292 A phage library of cDNA from Arabidopsis thaliana has been screened with oligodeoxyribonucleotides designed from regions of high homology found in tobacco osmotin and other osmotin-like proteins. One of the selected clones, Atosm34, presents a 734 bp open reading frame encoding a polypeptide of 244 amino acids, including the putative N-terminal signal and C-terminal propeptide sequences. Comparative alignment reveals extensive homologies to osmotin and the osmotin-like proteins found in Solanaceae, and also to a related polypeptide found in soybean. Genomic hybridization suggests that the cDNA obtained here corresponds to a single copy gene, and RNA blot analysis showed that the level of expression is highest in old leaves. Pathogenesis-related protein Osmotic stress-related protein Thaumatin-like protein Dewey 580 45 Département des sciences végétales 163 false Gene 191 1 1bca2b53-d461-4187-86b3-64d618b07677 Article (Published version) Restricted 1-s2.0-S0378111997000292-main-1.pdf 429525 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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Isolation and characterization of a cDNA clone encoding an osmotin-like protein from Arabidopsis thaliana	Gene			1997	293	0

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Diogon, Thierry Robert René