Photoreceptor-induced sinapate synthesis contributes to photoprotection in Arabidopsis

Article Article scientifique Article Photoreceptor-induced sinapate synthesis contributes to photoprotection in Arabidopsis eng 2024-06-26 2024-10-01 author Leonardelli Manuela 0000-0001-6215-1478 Department of Plant Sciences, Section of Biology, Faculty of Sciences, University of Geneva, CH-1211 Geneva, Switzerland. 905522 author Tissot Nicolas 0000-0003-3411-894X Department of Plant Sciences, Section of Biology, Faculty of Sciences, University of Geneva, CH-1211 Geneva, Switzerland. 876502 author Podolec Roman 0000-0003-2998-7892 Department of Plant Sciences, Section of Biology, Faculty of Sciences, University of Geneva, CH-1211 Geneva, Switzerland.,Institute of Genetics and Genomics in Geneva (iGE3), University of Geneva, CH-1211 Geneva, Switzerland. 747840 author Ares-Orpel Florence Department of Plant Sciences, Section of Biology, Faculty of Sciences, University of Geneva, CH-1211 Geneva, Switzerland. 588234 author Glauser Gaétan 0000-0002-0983-8614 Neuchâtel Platform of Analytical Chemistry, University of Neuchâtel, CH-2000 Neuchâtel, Switzerland. author Ulm Roman 0000-0001-8014-7392 Department of Plant Sciences, Section of Biology, Faculty of Sciences, University of Geneva, CH-1211 Geneva, Switzerland.,Institute of Genetics and Genomics in Geneva (iGE3), University of Geneva, CH-1211 Geneva, Switzerland. 732804 Département des sciences végétales (BIVEG) author Demarsy Emilie 0000-0002-0638-0812 Department of Plant Sciences, Section of Biology, Faculty of Sciences, University of Geneva, CH-1211 Geneva, Switzerland. 867450 Département des sciences végétales (BIVEG) 1518-1533 10.1093/plphys/kiae352 38918833 0032-0889 PMC11444301 https://academic.oup.com/plphys/article/196/2/1518/7699120 Plants must balance light capture for photosynthesis with protection from potentially harmful ultraviolet (UV) radiation. Photoprotection is mediated by concerted action of photoreceptors, but the underlying molecular mechanisms are not fully understood. In this study, we provide evidence that UV RESISTANCE LOCUS 8 (UVR8) UV-B, phytochrome red, and cryptochrome blue-light photoreceptors converge on the induction of FERULIC ACID 5-HYDROXYLASE 1 (FAH1) that encodes a key enzyme in the phenylpropanoid biosynthesis pathway, leading to the accumulation of UV-absorbing sinapate esters in Arabidopsis (Arabidopsis thaliana). FAH1 induction depends on the basic leucine zipper transcription factors ELONGATED HYPOCOTYL 5 (HY5) and HY5 HOMOLOG that function downstream of all 3 photoreceptors. Noticeably, mutants with hyperactive UVR8 signaling rescue fah1 UV sensitivity. Targeted metabolite profiling suggests that this phenotypic rescue is due to the accumulation of UV-absorbing metabolites derived from precursors of sinapate synthesis, namely, coumaroyl glucose and feruloyl glucose. Our genetic dissection of the phenylpropanoid pathway combined with metabolomic and physiological analyses show that both sinapate esters and flavonoids contribute to photoprotection with sinapates playing a major role for UV screening. Our findings indicate that photoreceptor-mediated regulation of FAH1 and subsequent accumulation of sinapate "sunscreen" compounds are key protective mechanisms to mitigate damage, preserve photosynthetic performance, and ensure plant survival under UV. Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Arabidopsis / genetics Arabidopsis / metabolism Arabidopsis / radiation effects Basic-Leucine Zipper Transcription Factors / genetics Basic-Leucine Zipper Transcription Factors / metabolism Chromosomal Proteins, Non-Histone Coumaric Acids / metabolism Gene Expression Regulation, Plant Malates Mixed Function Oxygenases / genetics Mixed Function Oxygenases / metabolism Mutation Phenylpropionates Photoreceptors, Plant / genetics Photoreceptors, Plant / metabolism Ultraviolet Rays Dewey 590 Dewey 580 Swiss National Science Foundation Perception and signalling of UV-B radiation in plants 175774 CH Projects Swiss National Science Foundation Perception and signalling of UV-B radiation in plants 207716 CH Projects 45 Département des sciences végétales 163 23 Institut de génétique et de génomique 0DH false 832 Groupe Ulm Plant physiology 196 2 4e199821-f579-4f27-a2f1-e75e11575f47 Article (Published version) Public Article_scientifique_from_europePmc.pdf 8161275 application/pdf CC BY-NC-ND-4.0 2f329a2414b116da2dfad41f3c491175dd73baba5ba02b44b3e446e17f059987 ebd5859a-276b-4264-b1ab-983c853b2bae Appendix Public Leonardelli_Plantphys_2024_rev2_Sup.pdf 1136238 application/pdf Supplementary Data 350e975b6bdc65516fc14d21a9f1e0fdeca0d498bc144ed9418542195aacd8e8 2518c35c-830c-4907-a0f2-dc2b1fbff6c4 Appendix Public pp.00317.2024-s03.pdf 348055 application/pdf Peer-Review report 31de9c6767d64e7405c06a918635b58dfdc2032267a88f1cb87ca015d3dbebbd openAccess Faculté des sciences / Section de biologie / Département des sciences végétales Centres et instituts / Institut de génétique et de génomique

Title	Published in	Year	Views	Downloads

Photoreceptor-induced sinapate synthesis contributes to photoprotection in Arabidopsis	Plant physiology	2024	23	159
Arabidopsis B‐box transcription factors BBX20 ‐22 promote UVR8 photoreceptor‐mediated UV‐B responses	Plant journal	2022	206	325

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Leonardelli, Manuela