Comparison against current standards of a DNA aptamer for the label-free quantification of tobramycin in human sera employed for therapeutic drug monitoring

Article Article scientifique Article Comparison against current standards of a DNA aptamer for the label-free quantification of tobramycin in human sera employed for therapeutic drug monitoring eng 2018 author Tenaglia Enrico 899437 author Ferretti Anna author Decosterd Laurent 826211 author Werner Dominique author Mercier Thomas author Widmer Nicolas 0000-0001-7392-2418 604325 author Buclin Thierry 648525 author Guiducci Carlotta 341-347 10.1016/j.jpba.2018.06.061 30025299 0731-7085 The use of DNA aptamers in biosensors for the quantification of pharmaceuticals in the clinics would help to overcome the limitations of antibody-based detection for small molecules. The interest for such systems is proven by the ever-increasing number of aptamer-based solutions for analytics proposed in the literature as proof-of-concept demonstrators. Despite such diversity, these platforms often lack a comparative assessment of their performances against the current standard of practice in the clinics when using real samples. We employed an aptamer against tobramycin discovered in our laboratory to quantify through surface plasmon resonance the concentration of the antibiotic in clinical samples obtained from patients treated with tobramycin and undergoing therapeutic drug monitoring. We then compared the performances of our detection strategy against the current standard of practice. Our results show how, using adequate calibration and matrix complexity reduction, DNA aptamer-based direct assays can assess clinically relevant concentrations of small molecules in patient serum and with good correlation to current standards used in the clinics. Anti-Bacterial Agents/blood Aptamers, Nucleotide/blood Drug Monitoring/methods/standards Humans Surface Plasmon Resonance Tobramycin/blood Dewey 615 31 Section des sciences pharmaceutiques 17 false 887 Pharmacie hospitalière et clinique Journal of Pharmaceutical and Biomedical Analysis 159 d0790ec8-f657-4108-b5c1-4d50f489f0b8 Article (Published version) Restricted Aptamer_tobramycine.pdf 1699447 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

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Comparison against current standards of a DNA aptamer for the label-free quantification of tobramycin in human sera employed for therapeutic drug monitoring	Journal of Pharmaceutical and Biomedical Analysis			2018	240	2

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Tenaglia, Enrico