Three-dimensional chemotaxis-driven aggregation of tumor cells

Article Article scientifique Article Three-dimensional chemotaxis-driven aggregation of tumor cells eng 2015-10-16 2015-10-16 author Puliafito Alberto 0000-0002-0096-1536 author De Simone Alessandro 0000-0002-1165-8014 985726 Département de génétique et évolution (Genev) (BIANI) author Seano Giorgio 0000-0002-7294-302X author Gagliardi Paolo Armando 0000-0002-4818-035X author Di Blasio Laura 0000-0002-1337-7654 author Chianale Federica author Gamba Andréa Alexander 0000-0002-2591-2722 89827 author Primo Luca 0000-0002-1294-0441 author Celani Antonio 0000-0002-0339-1073 1-12 15205 10.1038/srep15205 26471876 2045-2322 PMC4607978 https://www.nature.com/articles/srep15205 <p>One of the most important steps in tumor progression involves the transformation from a differentiated epithelial phenotype to an aggressive, highly motile phenotype, where tumor cells invade neighboring tissues. Invasion can occur either by isolated mesenchymal cells or by aggregates that migrate collectively and do not lose completely the epithelial phenotype. Here, we show that, in a three-dimensional cancer cell culture, collective migration of cells eventually leads to aggregation in large clusters. We present quantitative measurements of cluster velocity, coalescence rates and proliferation rates. These results cannot be explained in terms of random aggregation. Instead, a model of chemotaxis-driven aggregation – mediated by a diffusible attractant – is able to capture several quantitative aspects of our results. Experimental assays of chemotaxis towards culture conditioned media confirm this hypothesis. Theoretical and numerical results further suggest an important role for chemotactic-driven aggregation in spreading and survival of tumor cells.</p> true Scientific reports 5 1 6f363bac-ae63-4245-926e-c3370cb93d67 Article (Published version) Public Article-scientifique-from-unpaywall.pdf 2569674 application/pdf CC BY-4.0 ab6aeddb8c3471f79305f913107b3d5c7d0e7f5f0e1a58f9038764e53d7695c3 openAccess

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Three-dimensional chemotaxis-driven aggregation of tumor cells	Scientific reports			2015	64	34

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Gamba, Andréa Alexander