Phosphorylated B<sub>6</sub> vitamer deficiency in SALT OVERLY SENSITIVE 4 mutants compromises shoot and root development

Article Article scientifique Article Phosphorylated B<sub>6</sub> vitamer deficiency in SALT OVERLY SENSITIVE 4 mutants compromises shoot and root development eng 2022-01-20 author Gorelova Vera Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland. 895641 author Colinas Martinez Maite Felicitas 0000-0001-7053-2983 Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland. 728205 author Dell'Aglio Elisa Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland. 827245 author Flis Paulina 0000-0002-5529-7599 Future Food Beacon of Excellence and School of Biosciences, University of Nottingham, Leicestershire LE12 5RD, UK. author Salt David E 0000-0003-0283-0991 Future Food Beacon of Excellence and School of Biosciences, University of Nottingham, Leicestershire LE12 5RD, UK. author Fitzpatrick Thérésa Bridget 0000-0001-7694-5631 Department of Botany and Plant Biology, University of Geneva, 1211 Geneva, Switzerland. 684438 Département des sciences végétales (BIVEG) 220-240 10.1093/plphys/kiab475 34730814 0032-0889 PMC8774746 Stunted growth in saline conditions is a signature phenotype of the Arabidopsis SALT OVERLY SENSITIVE mutants (sos1-5) affected in pathways regulating the salt stress response. One of the mutants isolated, sos4, encodes a kinase that phosphorylates pyridoxal (PL), a B6 vitamer, forming the important coenzyme pyridoxal 5'-phosphate (PLP). Here, we show that sos4-1 and more recently isolated alleles are deficient in phosphorylated B6 vitamers including PLP. This deficit is concomitant with a lowered PL level. Ionomic profiling of plants under standard laboratory conditions (without salt stress) reveals that sos4 mutants are perturbed in mineral nutrient homeostasis, with a hyperaccumulation of transition metal micronutrients particularly in the root, accounting for stress sensitivity. This is coincident with the accumulation of reactive oxygen species, as well as enhanced lignification and suberization of the endodermis, although the Casparian strip is intact and functional. Further, micrografting shows that SOS4 activity in the shoot is necessary for proper root development. Growth under very low light alleviates the impairments, including salt sensitivity, suggesting that SOS4 is important for developmental processes under moderate light intensities. Our study provides a basis for the integration of SOS4 derived B6 vitamers into plant health and fitness. Arabidopsis / genetics Arabidopsis / growth & development Gene Expression Regulation, Plant Genes, Plant Genetic Variation Genotype Mutation Organogenesis, Plant / genetics Plant Roots / genetics Plant Roots / growth & development Plant Shoots / genetics Plant Shoots / growth & development Pyridoxal Phosphate / genetics Pyridoxal Phosphate / metabolism Salt Stress / genetics Salt Tolerance / genetics Dewey 580 45 Département des sciences végétales 163 false Plant physiology 188 1 a21aee27-5b90-47e4-9f38-92f2d6a85959 Article (Published version) Restricted Article-scientifique-from-unpaywall.pdf 2577058 application/pdf 21691830ba0aaddc536d9f278b25e7eb70f8e51dd3649df0c026b0ca3958cd9a restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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Phosphorylated B₆ vitamer deficiency in SALT OVERLY SENSITIVE 4 mutants compromises shoot and root development	Plant physiology			2022	83	0

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Gorelova, Vera