Topology changes of Hydra define actin orientation defects as organizers of morphogenesis

Article Article scientifique Article Topology changes of Hydra define actin orientation defects as organizers of morphogenesis eng 2025-01-17 author Ravichandran Yamini Yogalakshmi 0000-0003-3123-1242 Department of Biochemistry, Université de Genève, CH-1211 Genève, Switzerland. 966638 Département de biochimie (CHIBI) author Vogg Matthias Christian Department of Genetics & Evolution, Université de Genève, CH-1211 Genève, Switzerland. 831155 author Kruse Karsten 0000-0001-8665-2349 Department of Biochemistry, Université de Genève, CH-1211 Genève, Switzerland.,Department of Theoretical Physics, Université de Genève, CH-1211 Genève, Switzerland. 861969 Département de biochimie (CHIBI) author Pearce Daniel 0000-0002-0223-3778 Department of Theoretical Physics, Université de Genève, CH-1211 Genève, Switzerland. 894478 Département de physique théorique (FITEO) author Roux Aurélien 0000-0002-6088-0711 Department of Biochemistry, Université de Genève, CH-1211 Genève, Switzerland. 716946 Département de biochimie (CHIBI) eadr9855 10.1126/sciadv.adr9855 39823327 2375-2548 PMC11740953 https://www.science.org/doi/10.1126/sciadv.adr9855 Hydra regenerates one head when cut, but how forces shaping the head are coordinated remains unclear. Soft compression of Hydra 's head-regenerating tissues induces the formation of viable, two-headed animals. Compression creates new topological defects in the supracellular orientational order of muscular actin fibers, associated with additional heads. Theory supports that these defects organize muscle stresses required to shape the head. By compressing head-regenerating tissues along their body axis, we formed toroidal tissues, whose unique topology allows for the absence of defects. Toroids with no actin defects did not regenerate. Toroids with actin defects regenerated into viable toroidal animals with a bifurcated body. Topological defects in the actin orientational order are thus necessary for complete regeneration of Hydra , defining actin topological defects as mechanical organizers of morphogenesis. Dewey 540 Dewey 500.2 Dewey 590 Swiss National Science Foundation Controlling active materials with topological defects 211367 CH Programmes European Commission In-situ spatiotemporal imaging of membrane hydration, electrostatics, tension and curvature to understand cell response to osmotic shocks and cell migration. 951324 R2-TENSION EU H2020 39 Département de biochimie 144 37 Département de physique théorique 133 44 Département de génétique et évolution 162 false 769 Groupe Roux 1402 Groupe Kruse Science advances 11 3 7f371b6c-b273-45cd-aa8c-72898e5da997 Article (Published version) Public sciadv.adr9855.pdf 6682817 application/pdf CC BY-4.0 2c4bceb8806ebfd574457a10998964ac2f3f213c53d5037b35dc3b45e6893e80 openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté des sciences / Section de physique / Département de physique théorique Faculté des sciences / Section de biologie / Département de génétique et évolution

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Topology changes of Hydra define actin orientation defects as organizers of morphogenesis	Science advances			2025	205	159

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