Cytoplasmic flow is a cell size sensor that scales anaphase

Article Article scientifique Article Cytoplasmic flow is a cell size sensor that scales anaphase eng 2025-01-31 author Martins Afonso E Silva Olga Sofia 0000-0002-5054-2460 Department of Biochemistry, Faculty of Sciences, University of Geneva, Geneva, Switzerland. olga.afonso@unige.ch. 892586 Biochimie groupe 6 (CHIB6) author Dumoulin Ludovic Department of Biochemistry, Faculty of Sciences, University of Geneva, Geneva, Switzerland.,Department of Theoretical Physics, Faculty of Sciences, University of Geneva, Geneva, Switzerland. 935142 Biochimie (groupe 10) (CHIB10) author Kruse Karsten 0000-0001-8665-2349 Department of Biochemistry, Faculty of Sciences, University of Geneva, Geneva, Switzerland.,Department of Theoretical Physics, Faculty of Sciences, University of Geneva, Geneva, Switzerland. 861969 Département de biochimie (CHIBI) author Gonzalez Gaitan Marcos 0000-0002-6295-7321 Department of Biochemistry, Faculty of Sciences, University of Geneva, Geneva, Switzerland. marcos.gonzalez@unige.ch. 645929 Département de biochimie (CHIBI) 10.1038/s41556-024-01605-6 39890956 1465-7392 https://www.nature.com/articles/s41556-024-01605-6 During early embryogenesis, fast mitotic cycles without interphase lead to a decrease in cell size, while scaling mechanisms must keep cellular structures proportional to cell size. For instance, as cells become smaller, if the position of nuclear envelope reformation (NER) did not adapt, NER would have to occur beyond the cell boundary. Here we found that NER position in anaphase scales with cell size via changes in chromosome motility, mediated by cytoplasmic flows that themselves scale with cell size. Flows are a consequence of friction between viscous cytoplasm and bulky cargo transported by dynein on astral microtubules. As an emerging property, confinement in cells of different sizes yields scaling of cytoplasmic flows. Thus, flows behave like a cell geometry sensor: astral microtubules approach the boundary causing flow velocity changes, which then affect the velocity of chromosome separation, thus scaling NER. Dewey 540 Dewey 500.2 39 Département de biochimie 144 37 Département de physique théorique 133 false 765 Groupe Gonzalez Gaitan 1402 Groupe Kruse Nature cell biology d0b7edee-f85a-4290-b87e-79f2574694e4 Article (Published version) Public Article_scientifique_from_crossref.pdf 13678808 application/pdf CC BY-4.0 915bbd5f6eacce9fb256743f9e4b92f45f3cc44a079e67322c9e40d033152c18 openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté des sciences / Section de physique / Département de physique théorique

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Cytoplasmic flow is a cell size sensor that scales anaphase	Nature cell biology			2025	105	198

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Martins Afonso E Silva, Olga Sofia

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