TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus

Article Article scientifique Article TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus eng 1999 author Braun Isabelle Caroline 88828 author Rohrbach Emmanuelle 126230 author Schmitt Christel 506592 author Izaurralde Elisa 22473 1953-1965 10.1093/emboj/18.7.1953 0261-4189 http://emboj.embopress.org/cgi/doi/10.1093/emboj/18.7.1953 The constitutive transport element (CTE) of the simian type D retroviruses overcomes nuclear retention and allows nuclear export of unspliced viral RNAs by recruiting TAP, a host factor which is thought to be required for export of cellular mRNAs. In this report, we show that the first 372 amino acid residues of TAP, comprising a stretch of leucine‐rich repeats, are both necessary and sufficient for binding to the CTE RNA and promoting its export to the cytoplasm. Moreover, like the full‐length protein, this domain migrates to the cytoplasm upon nuclear co‐injection with the CTE RNA. Together, these results indicate that the CTE‐binding domain includes the signals for nuclear export. We also describe a derivative of TAP that bears a triple amino acid substitution within the CTE‐binding domain and substantially reduces the export of mRNAs from the nucleus. This provides further evidence for a role for TAP in this process. Thus, the CTE‐binding domain of TAP defines a novel RNA‐binding motif which has dual functions, both recognizing the CTE RNA and interacting with other components of the nuclear transport machinery. CTE Leucine-rich repeats mRNA export Nuclear export RNA-protein interactions Dewey 570 43 Département de biologie moléculaire 161 false EMBO Journal 18 7 8c266a57-9df2-41ee-9742-910ef453ced1 Article (Published version) Restricted Braun_et_al-1999-The_EMBO_Journal.pdf 516075 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus	EMBO Journal			1999	291	0

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Braun, Isabelle Caroline