Hyperoxia-mediated oxidative stress increases expression of UCP3 mRNA and protein in skeletal muscle

Article Article scientifique Article Hyperoxia-mediated oxidative stress increases expression of UCP3 mRNA and protein in skeletal muscle eng 2005 author Flandin-Blety Pierre François 88753 author Donati Yves Richard 24165 author Barazzone Constance 29124 author Muzzin Patrick 23003 3411-3415 10.1016/j.febslet.2005.04.084 15922330 0014-5793 The uncoupling protein-3 (UCP3) is a mitochondrial protein expressed mainly in skeletal muscle. Among several hypotheses for its physiological function, UCP3 has been proposed to prevent excessive production of reactive oxygen species. In the present study, we evaluated the effect of an oxidative stress induced by hyperoxia on UCP3 expression in mouse skeletal muscle and C2C12 myotubes. We found that the hyperoxia-mediated oxidative stress was associated with a 5-fold and 3-fold increase of UCP3 mRNA and protein levels, respectively, in mouse muscle. Hyperoxia also enhanced reactive oxygen species production and UCP3 mRNA expression in C2C12 myotubes. Our findings support the view that both in vivo and in vitro UCP3 may modulate reactive oxygen species production in response to an oxidative stress. Animals Carrier Proteins/genetics/metabolism Cells, Cultured Ion Channels Mice Mitochondrial Proteins Muscle Fibers, Skeletal/chemistry/metabolism Muscle, Skeletal/chemistry/metabolism Oxidative Stress/genetics/physiology RNA, Messenger/analysis/metabolism Reactive Oxygen Species/metabolism Dewey 616.07 Dewey 618 137 Département de pathologie et immunologie 21F 58 Département de pédiatrie, gynécologie et obstétrique 223 false 148 Facteurs influençants le développement pulmonaire: étude translationnelle chez l'animal et l'homme 182 FEBS letters 579 16 8841e03b-53d5-4e2d-843c-1cd13509b105 Article (Published version) Restricted 3411-3415.pdf 158744 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

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Hyperoxia-mediated oxidative stress increases expression of UCP3 mRNA and protein in skeletal muscle	FEBS letters			2005	567	0

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Flandin-Blety, Pierre François